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Literature summary extracted from

  • Ni, S.; Robinson, H.; Marsing, G.C.; Bussiere, D.E.; Kennedy, M.A.
    Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase from Shewanella oneidensis at 1.6 ANG: identification of farnesyl pyrophosphate trapped in a hydrophobic cavity (2004), Acta Crystallogr. Sect. D, D60, 1949-1957.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.12
-
Shewanella oneidensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.12 high-resolution structure, 16 A, of the enzyme in absence of substrate in the active site. Optimized crystals are obtained at a protein concentration of 35 mg/ml in a solution containing 4 M sodium formate and 5% glycerol. The crystals grow to avarage dimensions of 0.4 * 0.3 * 0.3 mM within a week Shewanella oneidensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.12 Na+ presence of tetrahedral Zn2+ in one of the metal-binding sites and an octahedral sodium ion in the second metal site in absence of substrate Shewanella oneidensis
4.6.1.12 Zn2+ presence of tetrahedral Zn2+ in one of the metal-binding sites and an octahedral sodium ion in the second metal site in absence of substrate Shewanella oneidensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.12 17300
-
3 * 17300 Shewanella oneidensis

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.12 Shewanella oneidensis
-
MR1
-
4.6.1.12 Shewanella oneidensis MR1
-
MR1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.12
-
Shewanella oneidensis

Subunits

EC Number Subunits Comment Organism
4.6.1.12 trimer 3 * 17300 Shewanella oneidensis

Synonyms

EC Number Synonyms Comment Organism
4.6.1.12 MECDP synthase
-
Shewanella oneidensis