Literature summary extracted from

Teartasin, W.; Limpkin, C.; Glod, F.; Spencer, J.; Cox, R.J.; Simpson, T.J.; Crosby, J.; Crump, M.P.; Hadfield, A.T.
Expression, purification and preliminary x-ray diffraction analysis of a ketoreductase from a type II polyketide synthase (2004), Acta Crystallogr. Sect. D, D60, 1137-1138.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.184	functional overexpression as His-tagged protein in Escherichia coli BL21(DE3)	Streptomyces coelicolor

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.184	5 mg/ml recombinant enzyme in 25 mM phosphate buffer, pH 8.0, with 10% glycerol, and 5 mM NADP+, hanging drop vapour diffusion method, against well solution containing 4.0-4.4 M sodium formate and 10% v/v glycerol, X-ray diffraction structure determination and analysis at 2.5 A resolution	Streptomyces coelicolor

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.184	Streptomyces coelicolor	-	actIII orf5 gene, ketoreductase type II	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.184	recombinant His-tagged active enzyme from Escherichia coli	Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.184	polyketid + NADPH	enzyme acts on the nascent polyketid chain from polyketid synthase reaction and reduces the carbonyl group at C-9	Streptomyces coelicolor	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.184	tetramer	in presence of NADPH, in absence of cofactor the enzyme forms mixtures of dimer, monomer, and tetramer, SDS-PAGE and native PAGE	Streptomyces coelicolor

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.184	ketoreductase	-	Streptomyces coelicolor
1.1.1.184	KR	-	Streptomyces coelicolor
1.1.1.184	More	enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes	Streptomyces coelicolor

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.184	7.3	-	assay at	Streptomyces coelicolor

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.184	NADPH	-	Streptomyces coelicolor

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Release 2023.1 (January 2023)