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Literature summary extracted from

  • Kondo, S.; Nakajima, Y.; Sugio, S.; Yong-Biao, J.; Sueda, S.; Kondo, H.
    Structure of the biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus at 2.2 A resolution (2004), Acta Crystallogr. Sect. D, 60, 486-492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.14 overexpression in Escherichia coli JM109 Aquifex aeolicus
6.4.1.1 expression in Escherichia coli JM109 Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.14 hanging-drop vapor-diffusion method, X-ray analysis Aquifex aeolicus
6.4.1.1 biotin carboxylase subunit Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 50000
-
 2 * 50000 Aquifex aeolicus
6.4.1.1 50000
-
 1 * 50000 + 1 * 70000 Aquifex aeolicus
6.4.1.1 70000
-
 1 * 50000 + 1 * 70000 Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 additional information Aquifex aeolicus BC subunit of pyruvate carboxylase, which is involved in gluconeogenesis ?
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- Aquifex aeolicus
-
 ADP + oxaloacetate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Aquifex aeolicus
-
-
-
6.4.1.1 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14 recombinant enzyme Aquifex aeolicus
6.4.1.1
-
 Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- BC subunit of pyruvate carboxylase, BC/active site structure Aquifex aeolicus ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 additional information BC subunit of pyruvate carboxylase, which is involved in gluconeogenesis Aquifex aeolicus ?
-
 ?
6.4.1.1 ATP + pyruvate + HCO3-
-
 Aquifex aeolicus ADP + oxaloacetate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 dimer 2 * 50000 Aquifex aeolicus
6.4.1.1 dimer 1 * 50000 + 1 * 70000 Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
6.3.4.14 PC-beta BC subunit of pyruvate carboxylase Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP structure of the ATP-binding site Aquifex aeolicus