EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.4.1 | medicine | enzyme is interesting for anticancer gene therapy since it deaminates the prodrug 5-fluorocytosine to the active drug 5-fluorouracil | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.1 | expression as His-tagged enzyme in Escherichia coli strain BL21 and as selenomethionine-labeled enzyme in strain B834 | Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.1 | purified recombinant wild-type and selenomethionine-labeled enzymes in 10% 2-propanol, 20% PEG 4000, 0.1 M Na HEPES, pH 7.5, at 4°C and at 22°C by hanging drop vapour diffusion method, 0.002 ml of both reservoir and protein solution are mixed in presence of 2-hydroxypyrimidine, 3-5 days to 1-2 weeks at 22°C, micro-seeding, X-ray diffraction structure determination and analysis at beyond 1.5 A resolution | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.1 | 2-hydroxypyrimidine | - |
Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.1 | 5-fluorocytosine + H2O | Saccharomyces cerevisiae | activation of the prodrug 5-fluorocytosine to the anticander drug 5-fluorouracil | 5-fluorouracil + NH3 | - |
? | |
3.5.4.1 | 5-methylcytosine + H2O | Saccharomyces cerevisiae | - |
thymine + NH3 | - |
? | |
3.5.4.1 | cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.1 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.1 | 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
3.5.4.1 | 5-fluorocytosine + H2O | activation of the prodrug 5-fluorocytosine to the anticander drug 5-fluorouracil | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
3.5.4.1 | 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
3.5.4.1 | cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? |