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Literature summary extracted from

  • Hsu, Y.H.; Hu, C.Y.; Lin, J.J.; Liaw, S.H.
    Crystallization and preliminary crystallographic analysis of yeast cytosine deaminase (2003), Acta Crystallogr. Sect. D, 59, 950-952.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.1 medicine enzyme is interesting for anticancer gene therapy since it deaminates the prodrug 5-fluorocytosine to the active drug 5-fluorouracil Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.1 expression as His-tagged enzyme in Escherichia coli strain BL21 and as selenomethionine-labeled enzyme in strain B834 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.1 purified recombinant wild-type and selenomethionine-labeled enzymes in 10% 2-propanol, 20% PEG 4000, 0.1 M Na HEPES, pH 7.5, at 4°C and at 22°C by hanging drop vapour diffusion method, 0.002 ml of both reservoir and protein solution are mixed in presence of 2-hydroxypyrimidine, 3-5 days to 1-2 weeks at 22°C, micro-seeding, X-ray diffraction structure determination and analysis at beyond 1.5 A resolution Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 2-hydroxypyrimidine
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O Saccharomyces cerevisiae activation of the prodrug 5-fluorocytosine to the anticander drug 5-fluorouracil 5-fluorouracil + NH3
-
?
3.5.4.1 5-methylcytosine + H2O Saccharomyces cerevisiae
-
thymine + NH3
-
?
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
uracil + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O
-
Saccharomyces cerevisiae 5-fluorouracil + NH3
-
?
3.5.4.1 5-fluorocytosine + H2O activation of the prodrug 5-fluorocytosine to the anticander drug 5-fluorouracil Saccharomyces cerevisiae 5-fluorouracil + NH3
-
?
3.5.4.1 5-methylcytosine + H2O
-
Saccharomyces cerevisiae thymine + NH3
-
?
3.5.4.1 cytosine + H2O
-
Saccharomyces cerevisiae uracil + NH3
-
?