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Literature summary extracted from

  • Zhao, H.; Bray, T.; Ouellette, M.; Zhao, M.; Ferre, R.A.; Matthews, D.; Whiteley, J.M.; Varughese, K.I.
    Structure of pteridine reductase (PTR1) from Leishmania tarentolae (2003), Acta Crystallogr. Sect. D, 59, 1539-1544.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.33 expression in Escherichia coli Leishmania tarentolae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.33 purified recombinant enzyme in binary complex with NADPH and in ternary complex with NADPH and methotrexate, 0.02 ml of protein solution containing 20 mg/ml protein, 20 mM Tris-HCl, pH 7.0, mixed with 0.01 ml of a solution containing 4 mM NADPH, 4 mM methotrexate on ice for 1 h, then mixed with 0.01 ml of reservoir solution containing 28% 1,4-butanediol, 12.5 mM cetyl trimethyl ammonium chloride and 100 mM HEPES, pH 7.0, 5 days, 14°C, X-ray diffraction structure determination and analysis of flash frozen crystals at 2.8 A resolution, molecular replacement technique Leishmania tarentolae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.33 methotrexate binding structure Leishmania tarentolae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.33 additional information Leishmania tarentolae enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Leishmania tarentolae P42556
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.33 recombinant enzyme from Escherichia coli Leishmania tarentolae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 Biopterin + NADPH 2-step reaction via intermediate 7,8-dihydrobiopterin Leishmania tarentolae 5,6,7,8-Tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 additional information enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway Leishmania tarentolae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.33 tetramer structure analysis Leishmania tarentolae

Synonyms

EC Number Synonyms Comment Organism
1.5.1.33 pteridine reductase
-
 Leishmania tarentolae
1.5.1.33 PTR1
-
 Leishmania tarentolae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.33 NADPH binding structure Leishmania tarentolae