Literature summary extracted from

Shirai, T.; Hung, V.S.; Akita, M.; Hatada, Y.; Ito, S.; Horikoshi, K.
Crystallization and preliminary X-ray study of alpha-glucosidase from Geobacillus sp strain HTA-462, one of the deepest sea bacteria (2003), Acta Crystallogr. Sect. D, 59, 1278-1279.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.20	10 mg/ml purified protein with 5 mM inhibitor D-(+)-glucono-1,5-lactone, hanging drop vapour diffusion method, 1 ml reservoir solution containing 20% v/v 2-propanol, 20% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, plus 0.01 ml of a solution consisting of 0.004 ml reservoir solution, 0.005 ml protein solution, containing 1% protein w/v in 10 mM phosphate, pH 7.0, and 0.001 ml inhibitor 50 mM, 2-3 weeks, X-ray diffraction structure determination at 2.5 A resolution	Geobacillus sp.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.20	D-glucono-1,5-lactone	-	Geobacillus sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.20	Geobacillus sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.20	to homogeneity by several step-procedure	Geobacillus sp.

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Release 2023.1 (January 2023)