Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Muench, S.P.; Rafferty, J.B.; McLeod, R.; Rice, D.W.; Prigge, S.T.
    Expression, purification and crystallization of the Plasmodium falciparum enoyl reductase (2003), Acta Crystallogr. Sect. D, 59, 1246-1248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expression in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 crystals are grown by hanging-drop vapour-diffusion, 0.0025 ml protein solution containing 12 mg/ml enzyme in 20 mM sodium/potassium phosphate, pH 8.0, 150 mM NaCl, 0.005 mM NAD+ and 0.006 mM triclosan are mixed with 0.0025 ml reservoir solution at 17°C, optimal reservoir solution contains 19.5% polyethylene glycol 3350 and 230 mM KI, crystals diffract to 2.2 A Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 triclosan
-
 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 recombinant His-tagged pfENR Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+
-
 Plasmodium falciparum trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 pfENR
-
 Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NAD+
-
 Plasmodium falciparum