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Literature summary extracted from

  • Jedrzejas, M.J.; Chantalat, L.
    Structural studies of streptococcus agalactiae hyaluronate lyase (2000), Acta Crystallogr. Sect. D, 56, 460-463.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.1 purified recombinant 111 kDa and 92 kDa enzyme forms, hanging drop vapour diffusion method, equal volumes of 0.001-0.005 ml of protein solution and reservoir solution, the latter containing 0.1 M cacodylate, pH 6.0, 30 mM KSCN, various amount of PEG-monomethyl ether 5000, over 1 ml reservoir solution, precipitation with PEG monomethyl ether 5000 and potassium thiocyanate at room temperature, X-ray diffraction structure determination and analysis at 2.1 A resolution Streptococcus agalactiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.1 92000
-
 autocatalytically processed enzyme, gel filtration Streptococcus agalactiae
4.2.2.1 111000
-
 pro-form, gel filtration Streptococcus agalactiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.1 additional information Streptococcus agalactiae the enzyme contributes to the invasive capacity of the pathogenic organism by degrading hyaluronan and chondroitin sulfates of the extracellular matrix of host tissues ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus agalactiae
-
 allele hylB3502
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.1 proteolytic modification autocatalytic conversion to a smaller 92kDa active enzyme form, completely prevented by 2 mM EDTA Streptococcus agalactiae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate also acts on chondroitin, the product is more systematically known as 3-(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose, catalytic mechanism Streptococcus agalactiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.1 3571
-
 purified 92 kDa mature form Streptococcus agalactiae
4.2.2.1 3680
-
 purified 111 kDa pro-form Streptococcus agalactiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 chondroitin sulfate
-
 Streptococcus agalactiae ?
-
 ir
4.2.2.1 hyaluronan
-
 Streptococcus agalactiae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ir
4.2.2.1 additional information the enzyme contributes to the invasive capacity of the pathogenic organism by degrading hyaluronan and chondroitin sulfates of the extracellular matrix of host tissues Streptococcus agalactiae ?
-
 ?