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Literature summary extracted from

  • Michaud, S.E.; Wang, L.Z.; Korde, N.; Bucki, R.; Randhawa, P.K.; Pastore, J.J.; Falet, H.; Hoffmeister, K.; Kuuse, R.; Uibo, R.; Herod, J.; Sawyer, E.; Janmey, P.A.
    Purification of salmon thrombin and its potential as an alternative to mammalian thrombins in fibrin sealants (2002), Thromb. Res., 107, 245-254.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.5 Ca2+ slight inhibition at 10 mM Homo sapiens
3.4.21.5 Ca2+ 50% inhibition at 10 mM Salmo salar

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.5 additional information not affected by NaCl up to 600 mM Homo sapiens
3.4.21.5 NaCl 50% activation at 600 mM Salmo salar

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.5 additional information Homo sapiens enzyme stimulates platelets and exposure of phosphatidylserine on the external surface ?
-
?
3.4.21.5 additional information Salmo salar enzyme stimulates platelets and exposure of phosphatidylserine on the external surface ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.5 Homo sapiens
-
-
-
3.4.21.5 Salmo salar
-
salmon
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.5 proteolytic modification activation of prothrombin by snake venom from Oxyuranus scutellatus Salmo salar

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.5 from plasma, to homogeneity Salmo salar

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.5 plasma
-
Salmo salar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.5 additional information
-
-
Salmo salar

Storage Stability

EC Number Storage Stability Organism
3.4.21.5 0°C, on ice, 8 h, stable Salmo salar
3.4.21.5 22°C, at room temperature, several hours, stable, lyophilized stable for several months Salmo salar

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.5 fibrinogen + H2O from human and salmon Salmo salar fibrin + fibrinopeptide A + fibrinopeptide B
-
?
3.4.21.5 additional information enzyme stimulates platelets and exposure of phosphatidylserine on the external surface Homo sapiens ?
-
?
3.4.21.5 additional information enzyme stimulates platelets and exposure of phosphatidylserine on the external surface Salmo salar ?
-
?
3.4.21.5 tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Homo sapiens tosyl-Gly-Pro-Arg + 4-nitroaniline
-
?
3.4.21.5 tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Salmo salar tosyl-Gly-Pro-Arg + 4-nitroaniline
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.5 30
-
-
Salmo salar
3.4.21.5 35
-
-
Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.5 80
-
lyophilized enzyme, 8 h, 90% remaining activity Homo sapiens
3.4.21.5 80
-
lyophilized purified enzyme, 8 h, 80% remaining activity Salmo salar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.5 8
-
-
Homo sapiens
3.4.21.5 8.5
-
-
Salmo salar