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Literature summary extracted from

  • Kiick, K.L.; Tirrell, D.A.
    Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase (2000), Tetrahedron, 56, 9487-9493.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 expression in an auxotroph Escherichia coli strain Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 (S)-2-amino-heptanoic acid L-methionine analogue, competitive Escherichia coli
6.1.1.10 (S)-2-aminohex-5-enoic acid L-methionine analogue, competitive Escherichia coli
6.1.1.10 (S)-2-aminohex-5-ynoic acid L-methionine analogue, competitive Escherichia coli
6.1.1.10 L-6,6,6-trifluoronorleucine L-methionine analogue, competitive Escherichia coli
6.1.1.10 L-cis-alpha-crotylglycine L-methionine analogue, competitive Escherichia coli
6.1.1.10 L-norleucine L-methionine analogue, competitive Escherichia coli
6.1.1.10 L-norvaline L-methionine analogue, competitive Escherichia coli
6.1.1.10 L-trans-alpha-crotylglycine L-methionine analogue, competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.0243
-
 L-methionine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 2.42
-
 (S)-2-aminohex-5-ynoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 4.12
-
 L-norleucine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 4.56
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 15.68
-
 (S)-2-aminohex-5-enoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Escherichia coli L-methionine and methionine analogue substrates are incorporated in proteins, overview AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Escherichia coli P00959
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.10 additional information
-
 incorporation level of L-methionine into proteins Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + (S)-2-aminohex-5-enoic acid + tRNAMet
-
 Escherichia coli AMP + diphosphate + (S)-2-aminohex-5-enoyl-tRNAMet
-
 ?
6.1.1.10 ATP + (S)-2-aminohex-5-enoic acid + tRNAMet 1850fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + (S)-2-aminohex-5-enoyl-tRNAMet
-
 ?
6.1.1.10 ATP + (S)-2-aminohex-5-ynoic acid + tRNAMet 500fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + 2(S)-aminohex-5-ynoyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet reduced activity with L-methionine analogues, overview, two-step reaction, the first step, the aminoacylation, is reversible, the seconde, the transfer reaction, is not Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet L-methionine and methionine analogue substrates are incorporated in proteins, overview Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-norleucine + tRNAMet 1050fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + L-norleucyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-trans-alpha-crotylglycine + tRNAMet 4700fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + L-trans-alpha-crotylglycyl-tRNAMet
-
 ?
6.1.1.10 additional information the enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Escherichia coli
6.1.1.10 Methionine--tRNA ligase
-
 Escherichia coli
6.1.1.10 Methionyl tRNA synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.10 MetRS
-
 Escherichia coli
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 1.35
-
 (S)-2-aminohex-5-enoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 1.82
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 2.15
-
 L-norleucine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 2.6
-
 (S)-2-aminohex-5-ynoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 2.94
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
6.1.1.10 13.3
-
 L-methionine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Escherichia coli