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Literature summary extracted from

  • Alexeev, D.; Baxter, R.L.; Campopiano, D.J.; McAlpine, R.S.; McIver, L.; Sawyer, L.
    Rational design of an inhibitor of dethiobiotin synthetase; interaction of 6-hydroxypyrimidin-4(3H)-one with the adenine base binding site (1998), Tetrahedron, 54, 15891-15898.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.3
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.3.3 crystals are grown from ammonium sulfate, crystals are soaked in the crystallisation-well solution plus 50 mM 6-hydroxypyrimidin-4(3H)-one for 1 hour, 6-hydroxypyrimidin-4(3H)-one is embedded in the base binding pocket of DTBS Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.3 6-hydroxypyrimidin-4(3H)-one competitive inhibition Escherichia coli
6.3.3.3 adenine
-
 Escherichia coli
6.3.3.3 AMP-PNP
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 Escherichia coli penultimate step in biotin biosynthesis ADP + phosphate + dethiobiotin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.3 recombinant DTBS, ammonium sulfate, Sephacryl S-200, Q-Sepharose Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 penultimate step in biotin biosynthesis Escherichia coli ADP + phosphate + dethiobiotin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.3 DTBS
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.3.3 0.05
-
 AMP-PNP 25°C, pH 7.8 Escherichia coli
6.3.3.3 2.2
-
 adenine 25°C, pH 7.8 Escherichia coli
6.3.3.3 11.2
-
 6-hydroxypyrimidin-4(3H)-one 25°C, pH 7.8 Escherichia coli