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Literature summary extracted from

  • Somoza, J.R.; Menon, S.; Schmidt, H.; Joseph-McCarthy, D.; Dessen, A.; Stahl, M.L.; Somers, W.S.; Sullivan, F.X.
    Structural and kinetic analysis of Escherichia coli GDP-mannose 4,6 dehydratase provides insights into the enzyme's catalytic mechanism and regulation by GDP-fucose (2000), Structure Fold. Des., 8, 123-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.47
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.47 hanging drop vapor diffusion method Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.47 K161A 3000fold reduced kcat Escherichia coli
4.2.1.47 T133V 3000fold reduced kcat Escherichia coli
4.2.1.47 Y157F 3000fold reduced kcat Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.47 GDP-fucose competitive inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.47 0.11
-
 GDP-mannose pH 7, 37°C, T133V mutant Escherichia coli
4.2.1.47 0.28
-
 GDP-mannose pH 7, 37°C, wild-type Escherichia coli
4.2.1.47 0.501
-
 GDP-mannose pH 7, 37°C, K161A mutant Escherichia coli
4.2.1.47 1.02
-
 GDP-mannose pH 7, 37°C, Y157F mutant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.47
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.47 GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 GDP-mannose
-
 Escherichia coli GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 dimer homodimer, gel filtration, equilibrium ultracentrifugation experiments Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.47 0.0015
-
 GDP-mannose pH 7, 37°C, T133V mutant Escherichia coli
4.2.1.47 0.0019
-
 GDP-mannose pH 7, 37°C, Y157F mutant Escherichia coli
4.2.1.47 0.0053
-
 GDP-mannose pH 7, 37°C, K161A mutant Escherichia coli
4.2.1.47 5
-
 GDP-mannose pH 7, 37°C, wild-type Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.47 NADP+ one NADP+ per monomer Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.47 0.0789
-
 GDP-fucose pH 7, 37°C Escherichia coli