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Literature summary extracted from

  • von Delft, F.; Lewendon, A.; Dhanaraj, V.; Blundell, T.L.; Abell, C.; Smith, A.G.
    The crystal structure of E. coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily (2001), Structure, 9, 439-450.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.1 native and selenomethionine labeled PS, hanging drop vapor diffusion, hanging drops are composed of equal volumes of PS and reservoir solutions consisting of 50 mM Tris-HCl, pH 8.0, 4%-6% polyethylene glycol 4000, crystals appeare at 19°C after 2-4 days, crystals diffract to 1.7 A Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.063
-
 (R)-pantoate
-
 Escherichia coli
6.3.2.1 0.1
-
 ATP
-
 Escherichia coli
6.3.2.1 0.15
-
 beta-Alanine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.1 30000
-
 2 * 30000, SDS-PAGE Escherichia coli
6.3.2.1 65000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine Escherichia coli
-
 AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1 recombinant pantothenate synthetase Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Escherichia coli AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 dimer 2 * 30000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 Pantothenate synthetase
-
 Escherichia coli
6.3.2.1 PS
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.1 10
-
-
 Escherichia coli