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Literature summary extracted from

  • Cusack, S.; Yaremchuk, A.; Krikliviy, I.; Tukalo, M.
    tRNAPro anticodon recognition by Thermus thermophilus prolyl-tRNA synthetase (1998), Structure, 6, 101-108.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.15 purified free enzyme or complexed with 3 tRNAPro isoacceptors, precipitant is 32% ammonium sulfate at pH 7.5, X-ray diffraction structure determination at 3.5 A resolution, and analysis Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Thermus thermophilus
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Thermus thermophilus
-
 purified class IIa enzyme
-
6.1.1.15 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 purified class IIa enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.15 ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro substrate binding mechanism, the two bases G35 and G36 are sufficient to uniquely identify the cognate tRNAPro Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Global RNA synthesis factor
-
 Thermus thermophilus
6.1.1.15 Pro-tRNA synthetase
-
 Thermus thermophilus
6.1.1.15 Proline translase
-
 Thermus thermophilus
6.1.1.15 Proline--tRNA ligase
-
 Thermus thermophilus
6.1.1.15 Prolyl RNA synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.15 ProRS
-
 Thermus thermophilus
6.1.1.15 ProRSTT
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Thermus thermophilus