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Literature summary extracted from

  • Baedeker, M.; Schulz, G.E.
    Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase (2002), Structure, 10, 61-67.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.3 expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.3 crystal structure of C273A/D145A, C273A/F329A and C273A/F329G double mutants at 2.25 A, 2.0 A and 1.9 A resolution, respectively Pseudomonas putida
4.3.1.3 crystal structure of native histidase inactivated with L-cystein at 1.0 A and Y280F mutant histidase at 1.7 A Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.3 A142D 3.4% of wild-type activity Pseudomonas putida
4.3.1.3 A142G 93% of wild-type activity Pseudomonas putida
4.3.1.3 C273A/D145A no activity, crystal structure Pseudomonas putida
4.3.1.3 C273A/F329A 0.04% of wild-type activity, crystal structure Pseudomonas putida
4.3.1.3 C273A/F329G no activity, crystal structure Pseudomonas putida
4.3.1.3 D145A no activity Pseudomonas putida
4.3.1.3 F329A 0.04% of wild-type activity Pseudomonas putida
4.3.1.3 F329G no activity Pseudomonas putida
4.3.1.3 G141A 1.1% of wild-type activity Pseudomonas putida
4.3.1.3 G144A 37% of wild-type activity Pseudomonas putida
4.3.1.3 Y280F 0.9% of wild-type activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 L-cysteine 10 mM, complete inactivation at pH higher than 10 in the presence of O2 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.3 L-histidine Pseudomonas putida
-
 urocanate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Pseudomonas putida P21310
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.3 recombinant histidase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine
-
 Pseudomonas putida urocanate + NH3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.3.1.3 HAL
-
 Pseudomonas putida