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Literature summary extracted from

  • Gilboa, R.; Spungin-Bialik, A.; Wohlfahrt, G.; Schomburg, D.; Blumberg, S.; Shoham, G.
    Interactions of Streptomyces griseus aminopeptidase with amino acid reaction products and their implications toward a catalytic mechanism (2001), Proteins, 44, 490-504.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.24 biotechnology enzyme is attractive for diverse applications, e.g. the processing of recombinant DNA proteins and fusion protein production due to its heat stability, high activity, and small size Streptomyces griseus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.24 purified enzyme complexed with L-methionine, L-phenylalanine, or L-leucine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 100 mM L-methionine or 200 mM L-leucine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml of reservoir precipitant solution, 3-4 days, cyrstals of enzyme complexed with L-Phe were precipitated with 0.1 M acetate buffer, pH 5.5 instead in the same procedure within 8-10 weeks, X-ray complex structure determination at 1.6 A resolution and analysis Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 L-leucine binding mechanism and structure Streptomyces griseus
3.4.11.24 L-methionine binding mechanism and structure Streptomyces griseus
3.4.11.24 L-phenylalanine binding mechanism and structure Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Zn2+ double-zinc exopeptidase Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.24 release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues catalytic mechanism, high preference towards large hydrophobic amino terminus residues, active site structure, Glu131 is involved in the catalytic mechanism, enzyme-substrate and enzyme-product complex modeling Streptomyces griseus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?
3.4.11.24 peptide + H2O high preference towards large hydrophobic amino terminus residues Streptomyces griseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 M28.003 Merops-ID Streptomyces griseus
3.4.11.24 SGAP
-
 Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 8.4
-
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