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Literature summary extracted from

  • Kack, H.; Sandmark, J.; Gibson, K.J.; Schneider, G.; Lindqvist, Y.
    Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis (1998), Protein Sci., 7, 2560-2566.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.3.3 hanging-drop vapor diffusion, 0.005 ml of well solution containing 100 mM magnesium acetate or MgCl2, 9-11% polyethylene glycol 8000 and 100 mM cacodylate, pH 6.5 are mixed with 0.002 ml protein solution containing 30 mg/ml DTBS, crystals grow within a week at 20°C, crystals of DTBS complexed with diaminopelargonic acid-MgADP-AlF3 and with dethiobiotin-MgADP-phosphate, crystals diffract to 1.8 A Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 Escherichia coli penultimate step in biotin biosynthesis ADP + phosphate + dethiobiotin
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 penultimate step in biotin biosynthesis Escherichia coli ADP + phosphate + dethiobiotin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.3 DTBS
-
 Escherichia coli