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Literature summary extracted from

  • Aertgeerts, K.; Ye, S.; Tennant, M.G.; Kraus, M.L.; Rogers, J.; Sang, B.C.; Skene, R.J.; Webb, D.R.; Prasad, G.S.
    Crystal structure of human dipeptidyl peptidase IV in complex with a decapeptide reveals details on substrate specificity and tetrahedral intermediate formation (2004), Protein Sci., 13, 412-421.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 expression of soluble His-tagged enzyme wild-type and a selenomethionine forms, which comprise extracellular domain amino acid residues 39-766 and are secreted to the medium, in Trichoplusia ni Hi5 insect cells via baculovirus infection Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.14.5 crystal structure of the free form of DPPIV and of the enzyme in complex with the first 10 residues of the physiological substrate neuropeptide Y (tNPY). The structure of the enzyme/tNPY complex suggests that bioactive peptides utilize the side opening unique to DPPIV to access the active site. Space group of wild-type enzyme and enzyme/tNPY complex is P2(1). The cell dimensions for wild-type enzyme are a = 121.8 A, b = 124,1 A, c = 1445 A, beta = 114.7°. The cell dimensions for the enzyme /tNPY complex are a = b = 122.5 A, c = 145.3 A, beta = 114.9° Homo sapiens
3.4.14.5 purified recombinant enzyme extracellular domain, wild-type or selenomethionine-form, both free, and the wild-type also complexed with the decapeptide YPSKPDNPGE, corresponding to the first 10 amino acids of neuropeptide Y, 4°C, nanovolume crystallization, over reservoir solution containing 20% PEG monomethyl ester 2000, 100 mM bicine, pH 8.0-8.5, 5 days, X-ray diffraction structure determination and analysis at 2.1-2.3 A resolution, at 100 K using 25% v/v ethylene glycol as a cryoprotectant Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 glycoprotein N-glycosylation sites are N85, N92, N150, N219, N229, N281, N321, N520, N685, glycosylation is not important for catalytic activity, homodimer formation and adenosine deaminase binding Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5 recombinant soluble His-tagged wild-type and selenomethionine enzymes from insect cell culture supernatant to homogeneity Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.14.5 release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline prefers Pro or Ala at position P1, substrate recognition involves the negatively charged active site residues E205 and E206, reaction mechanism, active site structure and substrate binding Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 spleen
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Pro-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.14.5 additional information substrate specificity, enzyme binds adenosine deaminase Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer homodimer, crystal structure, not affected by glycosylation state, domain structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 ADABP
-
 Homo sapiens
3.4.14.5 adenosine deaminase binding protein
-
 Homo sapiens
3.4.14.5 CD26
-
 Homo sapiens
3.4.14.5 dipeptidyl peptidase IV
-
 Homo sapiens
3.4.14.5 DPPIV
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 22
-
 assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.4
-
 assay at Homo sapiens