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Literature summary extracted from
- N-linked glycosylation of dipeptidyl peptidase IV (CD26): effects on enzyme activity, homodimer formation, and adenosine deaminase binding (2004), Protein Sci., 13, 145-154.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.14.5 | expression of a soluble His-tagged wild-type enzyme and glycosylation mutant extracellular domains, which comprises extracellular domain amino acid residues 39-766 and are secreted to the medium, in Trichoplusia ni Hi5 and Spodoptera frugiperda Sf9 insect cells via baculovirus infection | Homo sapiens |
| 3.4.14.5 | expression of wild-type enzyme and mutant enzymes in Sf9 insect cells and in Hi5 insect cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | N150A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, 60% increased activity if expressed in Hi5 cells, unaltered activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N150A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is identical to of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 110% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N219A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, increased binding to adenosine deaminase of the mutant if expressed in Sf9 cells, increased activity if expressed in Hi5 cells, slightly altered activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N219A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 92% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 110% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N229A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, 90% increased activity if expressed in Hi5 cells, reduced activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N229A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 70% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 73% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N281A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, 40% increased activity if expressed in Hi5 cells, reduced activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N281A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 85% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 92% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N321A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, slightly increased binding to adenosine deaminase of the mutant if expressed in Sf9 cells, increased activity if expressed in Hi5 cells, reduced activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N321A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 50% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 54% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N520A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, slightly increased binding to adenosine deaminase of the mutant if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N520A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 68% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 80% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N685A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, slightly decreased binding to adenosine deaminase of the mutant if expressed in Hi5 cells | Homo sapiens |
| 3.4.14.5 | N685A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 110% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 120% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N85A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, slightly increased binding to adenosine deaminase of the mutant if expressed in Sf9 cells, increased activity if expressed in Hi5 cells, slightlyreduced activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N85A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is 85% of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 92% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
| 3.4.14.5 | N92A | site-directed mutagenesis, glycosylation site mutant, unaltered dimerization compared to the wild-type, slightly increased activity if expressed in Hi5 cells, unaltered activity if expressed in Sf9 cells | Homo sapiens |
| 3.4.14.5 | N92A | kcat/Km for mutant enzyme expressed in Sf9 insect cells is identical to of wild-type value for the substrate Ala-Pro-7-amido-4-trifluoromethylcoumarin and 110% of wild-type value for the substrate Gly-Pro-4-nitroanilide | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.5 | 0.013 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N685A, expressed in Sf9 cells | Homo sapiens |  |
| 3.4.14.5 | 0.013 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N685A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, wild-type soluble enzyme, mutant N92A, N150A, N229A, N281A, N321A and N520A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N150A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N229A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N281A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N321A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N520A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N92A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.014 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, wild-type enzyme expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.015 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N229A and N685A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.015 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N85A and N219A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.015 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N219A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.015 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N85A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.016 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, wild-type soluble enzyme and mutant N85A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.017 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N92A, N150A, and N520A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.018 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N321A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.019 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N219A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.02 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N281A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.131 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N520A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.138 | - |
Gly-Pro-4-nitroanilide | pH 7.4, wild-type soluble enzyme, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.142 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N520A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.142 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N685A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.143 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N150A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.143 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N92A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.144 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N219A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.145 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N229A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.146 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N321A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.147 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N92A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.149 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N85A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.151 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N150A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.151 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N229A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.152 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N685A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.155 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N85A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.156 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N321A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.159 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N281A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 0.16 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N219A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.168 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N281A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 0.17 | - |
Gly-Pro-4-nitroanilide | pH 7.4, wild-type soluble enzyme, expressed in Sf9 cells | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.14.5 | membrane | type II transmembrane protein | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 95000 | - |
2 * 95000, SDS-PAGE | Homo sapiens |
| 3.4.14.5 | 220000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | additional information | Homo sapiens | binding of adenosine deaminase to the T-cell surface protects the T-cell from adenosine-mediated inhibition of proliferation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.5 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | glycoprotein | 9 N-glycosylation sites, glycosylation is no prerequisite for enzyme activity, dimerization or binding of adenosine deaminase, structural analysis | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.5 | recombinant soluble His-tagged wild-type and mutant extracellular enzyme domains from insect cell culture supernatant to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | Ala-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.14.5 | Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | additional information | enzyme binds adenosine deaminase at the highly glycosylated beta-propeller domain | Homo sapiens | ? | - |
? | |
| 3.4.14.5 | additional information | binding of adenosine deaminase to the T-cell surface protects the T-cell from adenosine-mediated inhibition of proliferation | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | dimer | 2 * 95000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | ADABP | - |
Homo sapiens |
| 3.4.14.5 | adenosine deaminase binding protein | - |
Homo sapiens |
| 3.4.14.5 | CD26 | - |
Homo sapiens |
| 3.4.14.5 | dipeptidyl peptidase IV | - |
Homo sapiens |
| 3.4.14.5 | DPPIV | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.5 | 3 | 6 | Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N520A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 9 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N321A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 9 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N321A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 10 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N321A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 12 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N229A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 12 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N229A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 13 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N229A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 13 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N520A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 13 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N520A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 14 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N520A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 15 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N281A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 15 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N281A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 16 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N85A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 16 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N85A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 16 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N85A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 17 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N281A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 18 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, wild-type soluble enzyme, mutant N150A, N219A, and N92A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 18 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N150A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 18 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N219A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 18 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N92A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 18 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, wild-type enzyme expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 19 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N685A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 19 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N92A, N150A, and N219A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 19 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.4, mutant enzyme N685A expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 20 | - |
Gly-Pro-4-nitroanilide | pH 7.4, wild-type soluble enzyme, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 21 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N685A, expressed in Sf9 cells | Homo sapiens | |
| 3.4.14.5 | 23 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, wild-type soluble enzyme, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 24 | - |
Gly-Pro-4-nitroanilide | pH 7.4, wild-type soluble enzyme, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 27 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N85A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 30 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N685A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 30 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N85A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 31 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N92A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 32 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N685A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 34 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N321A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 34 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N92A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 35 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N321A and N520A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 37 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N150A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 40 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N150A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 40 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N229A and N281A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 42 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.4, mutant N219A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 43 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N229A and N281A, expressed in Hi5 cells | Homo sapiens | |
| 3.4.14.5 | 45 | - |
Gly-Pro-4-nitroanilide | pH 7.4, mutant N219A, expressed in Hi5 cells | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 7.4 | - |
assay at | Homo sapiens |
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