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Literature summary extracted from

  • Leiros, H.K.S.; Brandsdal, B.O.; Andersen, O.A.; Os, V.; Leiros, I.; Helland, R.; Otlewski, J.; Willassen, N.P.; Smalas, A.O.
    Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements (2004), Protein Sci., 13, 1056-1070.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.4 crystal structure of the enzyme-benzylamine complex, enzyme-phenylethylamine complex and the enzyme-phenylbutylamine complex Bos taurus
3.4.21.4 crystal structure of the enzyme-benzylamine complex, enzyme-phenylethylamine complex and the enzyme-phenylpropylamine complex salmon

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 benzylamine
-
Bos taurus
3.4.21.4 benzylamine
-
salmon
3.4.21.4 phenylbutylamine
-
Bos taurus
3.4.21.4 phenylbutylamine
-
salmon
3.4.21.4 Phenylethylamine
-
Bos taurus
3.4.21.4 Phenylethylamine
-
salmon
3.4.21.4 Phenylpropylamine
-
Bos taurus
3.4.21.4 Phenylpropylamine
-
salmon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.00017
-
tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 Bos taurus
3.4.21.4 0.000896
-
tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Bos taurus
-
-
-
3.4.21.4 salmon
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Bos taurus ?
-
?
3.4.21.4 tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
salmon ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 anionic salmon trypsin
-
salmon
3.4.21.4 AST
-
salmon
3.4.21.4 BT
-
Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 28
-
tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon
3.4.21.4 30.4
-
tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 salmon
3.4.21.4 88
-
tosyl-Gly-Pro-Arg-4-nitroanilide pH 8.3 Bos taurus