BRENDA - Enzyme Database

Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: Covalent versus noncovalent catalysis

Yang, H.; Roberts, M.F.; Protein Sci. 12, 2087-2098 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidic acid
up to 2fold
Streptomyces chromofuscus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces chromofuscus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
C123A
enzymatically inactive, secondary structure and thermostability similar to wild type
Streptomyces chromofuscus
3.1.4.4
C123S
enzymatically inactive, secondary structure and thermostability similar to wild type
Streptomyces chromofuscus
3.1.4.4
H171A
17% of wild type activity
Streptomyces chromofuscus
3.1.4.4
H187A
dramatic decrease in activity
Streptomyces chromofuscus
3.1.4.4
H200A
dramatic decrease in activity
Streptomyces chromofuscus
3.1.4.4
H266A
17% of wild type activity, activation by phosphatidic acid up to 12fold
Streptomyces chromofuscus
3.1.4.4
additional information
truncation of C-terminal 530 bp, no enzymic activtiy probably due o misfolding
Streptomyces chromofuscus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.16
-
1,2-dibutyl-sn-glycero-3-phosphocholine
pH 7.5, in presence of 5 mM Ca2+
Streptomyces chromofuscus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Streptomyces chromofuscus
Q8KRU5
ATCC type strain
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
recombinant enzyme
Streptomyces chromofuscus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
13
-
pH 7.5, in presence of 5 mM Ca2+
Streptomyces chromofuscus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
1,2-dibutyl-sn-glycero-3-phosphocholine + H2O
-
653805
Streptomyces chromofuscus
choline + ?
-
653805
Streptomyces chromofuscus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidic acid
up to 2fold
Streptomyces chromofuscus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces chromofuscus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
C123A
enzymatically inactive, secondary structure and thermostability similar to wild type
Streptomyces chromofuscus
3.1.4.4
C123S
enzymatically inactive, secondary structure and thermostability similar to wild type
Streptomyces chromofuscus
3.1.4.4
H171A
17% of wild type activity
Streptomyces chromofuscus
3.1.4.4
H187A
dramatic decrease in activity
Streptomyces chromofuscus
3.1.4.4
H200A
dramatic decrease in activity
Streptomyces chromofuscus
3.1.4.4
H266A
17% of wild type activity, activation by phosphatidic acid up to 12fold
Streptomyces chromofuscus
3.1.4.4
additional information
truncation of C-terminal 530 bp, no enzymic activtiy probably due o misfolding
Streptomyces chromofuscus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.16
-
1,2-dibutyl-sn-glycero-3-phosphocholine
pH 7.5, in presence of 5 mM Ca2+
Streptomyces chromofuscus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
recombinant enzyme
Streptomyces chromofuscus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
13
-
pH 7.5, in presence of 5 mM Ca2+
Streptomyces chromofuscus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
1,2-dibutyl-sn-glycero-3-phosphocholine + H2O
-
653805
Streptomyces chromofuscus
choline + ?
-
653805
Streptomyces chromofuscus
?