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Literature summary extracted from

  • Yang, H.; Roberts, M.F.
    Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: Covalent versus noncovalent catalysis (2003), Protein Sci., 12, 2087-2098.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 phosphatidic acid up to 2fold Streptomyces chromofuscus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4
-
 Streptomyces chromofuscus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 C123A enzymatically inactive, secondary structure and thermostability similar to wild type Streptomyces chromofuscus
3.1.4.4 C123S enzymatically inactive, secondary structure and thermostability similar to wild type Streptomyces chromofuscus
3.1.4.4 H171A 17% of wild type activity Streptomyces chromofuscus
3.1.4.4 H187A dramatic decrease in activity Streptomyces chromofuscus
3.1.4.4 H200A dramatic decrease in activity Streptomyces chromofuscus
3.1.4.4 H266A 17% of wild type activity, activation by phosphatidic acid up to 12fold Streptomyces chromofuscus
3.1.4.4 additional information truncation of C-terminal 530 bp, no enzymic activtiy probably due o misfolding Streptomyces chromofuscus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.16
-
 1,2-dibutyl-sn-glycero-3-phosphocholine pH 7.5, in presence of 5 mM Ca2+ Streptomyces chromofuscus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces chromofuscus Q8KRU5 ATCC type strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 recombinant enzyme Streptomyces chromofuscus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 13
-
 pH 7.5, in presence of 5 mM Ca2+ Streptomyces chromofuscus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 1,2-dibutyl-sn-glycero-3-phosphocholine + H2O
-
 Streptomyces chromofuscus choline + ?
-
 ?