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Literature summary extracted from

  • Wang, S.; Eisenberg, D.
    Crystal structures of a pantothenate synthetase from M. tuberculosis and its complexes with substrates and a reaction intermediate (2003), Protein Sci., 12, 1097-1108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.1 crystals of native PS and PS complexed with alpha,beta-methyleneadenosine 5'-triphosphate, pantoate and the reaction intermediate pantoyl adenylate, crystals are grown by hanging-drop vapor diffusion, 0.003-0.005 ml of PS solution at 10 mg/ml PS is mixed with an equal volume of well solution, best crystals are obtained with well solutions containing 10%-15% polyethylene glycol 3000, 5% glycerol, 2% ethanol, 20 mM MgCl2, 150 mM Li2SO4 and 100 mM imidazole, pH 8.0 at 20°C, crystals diffract to 1.6-2.0 A Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.1 E77G 87% of wild-type activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.1 31611
-
 2 * 31611, mass spectroscopy, crystallization Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine Mycobacterium tuberculosis
-
 AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1 recombinant PS Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 dimer 2 * 31611, mass spectroscopy, crystallization Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 panthotenate synthetase
-
 Mycobacterium tuberculosis
6.3.2.1 PS
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 1.74
-
 (R)-pantoate
-
 Mycobacterium tuberculosis
6.3.2.1 2.94
-
 (R)-pantoate
-
 Mycobacterium tuberculosis