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Literature summary extracted from

  • Chen, C.Y.; Lu, S.C.; Liao, T.H.
    The distinctive functions of the two structural calcium atoms in bovine pancreatic deoxyribonuclease (2002), Protein Sci., 11, 659-668.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.1 expression of wild-type and mutant enzymes in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.1 D201A site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and decrased Vmax compared to the wild-type enzyme, no double-scission ability, no protection by Ca2+ against trypsin inactivation Bos taurus
3.1.21.1 D99A site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and unaltered Vmax compared to the wild-type enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 Ca2+ slight inhibition of mutant D201A Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.21.1 additional information
-
 additional information
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ dependent on, D201 and D99 are involved in binding, conformation, protects wild-type and mutant D99A enzymes against trypsin inactivation, not mutant D201A Bos taurus
3.1.21.1 Mg2+ dependent on Bos taurus
3.1.21.1 Mn2+ activates Bos taurus
3.1.21.1 additional information metal binding and influence on activity, wild-type and mutant enzymes, overview Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 recombinant wild-type and mutant enzymes from Escherichia coli Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.21.1 endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 dsDNA + H2O wild-type enzyme and mutant D99A perform double scission on duplex DNA in presence of Mg2+ and Ca2+, not mutant D201A Bos taurus 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 pancreatic deoxyribonuclease
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7
-
 assay at Bos taurus