Literature summary extracted from
Chen, C.Y.; Lu, S.C.; Liao, T.H.
The distinctive functions of the two structural calcium atoms in bovine pancreatic deoxyribonuclease (2002), Protein Sci., 11, 659-668.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.21.1 |
expression of wild-type and mutant enzymes in Escherichia coli |
Bos taurus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.21.1 |
D201A |
site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and decrased Vmax compared to the wild-type enzyme, no double-scission ability, no protection by Ca2+ against trypsin inactivation |
Bos taurus |
3.1.21.1 |
D99A |
site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and unaltered Vmax compared to the wild-type enzyme |
Bos taurus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
Ca2+ |
slight inhibition of mutant D201A |
Bos taurus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
additional information |
- |
additional information |
- |
Bos taurus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
Ca2+ |
dependent on, D201 and D99 are involved in binding, conformation, protects wild-type and mutant D99A enzymes against trypsin inactivation, not mutant D201A |
Bos taurus |
|
3.1.21.1 |
Mg2+ |
dependent on |
Bos taurus |
|
3.1.21.1 |
Mn2+ |
activates |
Bos taurus |
|
3.1.21.1 |
additional information |
metal binding and influence on activity, wild-type and mutant enzymes, overview |
Bos taurus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.21.1 |
Bos taurus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.21.1 |
recombinant wild-type and mutant enzymes from Escherichia coli |
Bos taurus |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.21.1 |
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
preference for double-stranded DNA |
Bos taurus |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.21.1 |
pancreas |
- |
Bos taurus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.21.1 |
additional information |
- |
- |
Bos taurus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.21.1 |
dsDNA + H2O |
wild-type enzyme and mutant D99A perform double scission on duplex DNA in presence of Mg2+ and Ca2+, not mutant D201A |
Bos taurus |
5'-phosphodinucleotide + 5'-phosphooligonucleotide |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.21.1 |
pancreatic deoxyribonuclease |
- |
Bos taurus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.21.1 |
7 |
- |
assay at |
Bos taurus |