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Literature summary extracted from

  • Estevez, M.; Skarda, J.; Spencer, J.; Banaszak, L.; Weaver, T.M.
    X-ray crystallographic and kinetic correlation of a clinically observed human fumarase mutation (2002), Protein Sci., 11, 1552-1557.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.2 mutant enzyme E315Q Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.2 E315Q mutation causes about 3% increase in Km-value for S-malate, about 20% increase in Km-value for fumarate. 10fold decrease in turnover number for S-malate, about 11fold decrease in turnover number for fumarate Escherichia coli
4.2.1.2 additional information E319Q mutation, an inborn error of fumarase causes progressive psychomotor retardation, failure to thrive, microcephaly and abnormal posture with hypotonia contrasting with hypertonia of limbs Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.2 fumarate + H2O Homo sapiens enzyme of Krebs cycle L-malate
-
 r
4.2.1.2 fumarate + H2O Escherichia coli enzyme of Krebs cycle L-malate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Escherichia coli P05042
-
-
4.2.1.2 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 fumarate + H2O
-
 Homo sapiens L-malate
-
 r
4.2.1.2 fumarate + H2O
-
 Escherichia coli L-malate
-
 r
4.2.1.2 fumarate + H2O enzyme of Krebs cycle Homo sapiens L-malate
-
 r
4.2.1.2 fumarate + H2O enzyme of Krebs cycle Escherichia coli L-malate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.2 1
-
 L-malate pH 7.9, mutant enzyme E315Q Escherichia coli
4.2.1.2 1149
-
 fumarate pH 7.9, native enzyme Escherichia coli
4.2.1.2 1150
-
 fumarate pH 7.9, native enzyme Escherichia coli