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Literature summary extracted from

  • Snijder, H.J.; Van Eerde, J.H.; Kingma, R.L.; Kalk, K.H.; Dekker, N.; Egmond, M.R.; Dijkstra, B.W.
    Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytic triad (2001), Protein Sci., 10, 1962-1969.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 expression of N156A mutant Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.32 N156A mutant OMPLA is crystallized by hanging drop vapor-diffusion, drops consisting of 0.003 ml of protein solution and 0.002 ml reservoir solution are suspended over a 1 ml reservoir containing 27% 2-methyl-2,4-pentanediol, 0.4-1 mM CaCl2 and 100 mM Bis-Tris buffer, pH 6.0-6.3, the initial protein solution contained 10 mg/ml N156A OMPLA, 10 mM KCl, 1% 1-O-n-octyl-beta-D-glucopyranoside and 0.2 mM Tris-HCl at pH 6.6, crystals diffract to 2.5-3 A Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 outer membrane
-
 Escherichia coli 19867
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 Ca2+ required for activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O Escherichia coli
-
 2-acylglycerophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 N156A mutant Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O
-
 Escherichia coli 2-acylglycerophosphocholine + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 OMPLA
-
 Escherichia coli
3.1.1.32 outer membrane phospholipase A
-
 Escherichia coli