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Literature summary extracted from

  • Lauble, H.; Miehlich, B.; F๖rster, S.; Wajant, H.; Effenberger, F.
    Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from manihot esculenta in complex with acetone cyanohydrin (2001), Protein Sci., 10, 1015-1022.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.47 vapor diffusion hanging drop method, crystal structure of the mutant enzyme S80A refined to an R-factor of 18.0% against diffraction data to 2.1 A resolution, determination of three-dimensional structure of the complex of mutant enzyme S80A with acetone cyanohydrin at 2.2 A resultion, refined to an R-factor of 18.7% Manihot esculenta

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.47 C81A specific activity is 93% of the wild-type value Manihot esculenta
4.1.2.47 T11A specific activity is 24fold lower than that of the wild-type enzyme Manihot esculenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 169
-
 acetone cyanohydrin mutant enzyme C81A Manihot esculenta
4.1.2.47 174
-
 acetone cyanohydrin wild-type enzyme Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 2-hydroxy-2-methylpropanenitrile
-
 Manihot esculenta HCN + acetone
-
 ?