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Literature summary extracted from

  • Kulkarni, G.; Sabnis, N.A.; Harris, B.G.
    Cloning, expression, and purification of fumarase from the parasitic nematode Ascaris suum (2004), Protein Expr. Purif., 33, 209-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 expression in Escherichia coli BL21 Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 mesotartaric acid competitive Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 1.22
-
 (S)-malate pH 7.3, 30°C, recombinant enzyme Ascaris suum
4.2.1.2 1.43
-
 (S)-malate pH 7.3, 30°C, worm enzyme Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 50502
-
 x * 50502, calculation from nucleotide sequence Ascaris suum
4.2.1.2 51000
-
 x * 51000, SDS-PAGE Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Ascaris suum Q7Z1D9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Ascaris suum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 388
-
-
 Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Ascaris suum fumarate + H2O
-
 r
4.2.1.2 fumarate + H2O
-
 Ascaris suum L-malate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 ? x * 51000, SDS-PAGE Ascaris suum
4.2.1.2 ? x * 50502, calculation from nucleotide sequence Ascaris suum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.2 0.077
-
 mesotartaric acid pH 7.3, 30°C Ascaris suum