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Literature summary extracted from

  • Gasparian, M.E.; Ostapchenko, V.G.; Schulga, A.A.; Dolgikh, D.A.; Kirpichnikov, M.P.
    Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli (2003), Protein Expr. Purif., 31, 133-139.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.9 synthesis the enzyme can be used for cleavage of fusion proteins due to its high specific activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.9 expression of light chain of enteropeptidase in Escherichia coli BL21(DE3) in both soluble and insoluble form Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.9 Soybean trypsin inhibitor
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.9 0.14
-
thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 37°C Homo sapiens
3.4.21.9 0.16
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 Homo sapiens P98073 light chain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.9
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 fusion protein Trx/hEGF + H2O the fusion protein Trx/hEGF contains an inter-domain enteropeptidase recognition site Homo sapiens ?
-
?
3.4.21.9 fusion protein Trx/hIL-13 + H2O the fusion protein Trx/hIL-13 contains an inter-domain enteropeptidase recognition site Homo sapiens ?
-
?
3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
?
3.4.21.9 thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
Homo sapiens ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.9 115
-
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C Homo sapiens
3.4.21.9 133
-
thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 37°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.9 0.0000023
-
Soybean trypsin inhibitor pH 8.4, 37°C Homo sapiens