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Literature summary extracted from

  • Ni, S.X.; Cossar, D.; Man, A.; Norek, K.; Miller, D.; Kearse, C.; Tsvetnitsky, V.
    Purification and characterization of recombinant Streptomyces griseus aminopeptidase (2003), Protein Expr. Purif., 30, 62-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.24 expression in Streptomyces lividans by insertional cloning and protoplast transformation, the expression system contains the constitutive Streptomyces fradiae aph promoter Streptomyces griseus
3.4.11.24 gene SGAP, standard protoplast transformation and expression in Streptomyces lividans, excretion to the medium Streptomyces griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.24 extracellular
-
 Streptomyces griseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Zn2+ zinc-metallo-exoprotease Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.24 26800
-
 recombinant enzyme, gel filtration Streptomyces griseus
3.4.11.24 30800
-
 1 * 30800, recombinant enzyme, SDS-PAGE Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 recombinant enzyme from Streptomyces lividans cells by diafiltration, hydrophobic interaction chromatography, and gel filtration to homogeneity Streptomyces griseus
3.4.11.24 recombinant from Streptomyces lividans cell culture medium, high purity, 19% recovery Streptomyces griseus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.24 625.1
-
 purified enzyme Streptomyces griseus
3.4.11.24 625.1
-
 purified recombinant enzyme Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
3.4.11.24 -20°C, liquid and freeze-dried, stable for at least 8 weeks, no loss of activity Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 casein + H2O
-
 Streptomyces griseus ?
-
 ?
3.4.11.24 L-leucine-4-nitroanilide + H2O
-
 Streptomyces griseus L-leucine + 4-nitroaniline
-
 ?
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 monomer 1 * 30800, recombinant enzyme, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 M28.003 Merops-ID Streptomyces griseus
3.4.11.24 SGAP
-
 Streptomyces griseus
3.4.11.24 Streptomyces griseus aminopeptidase
-
 Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.24 24
-
 assay at Streptomyces griseus
3.4.11.24 37
-
 assay at Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.24 69
-
 recombinant enzyme, stable at for at least 1 h Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 7.8
-
 assay at Streptomyces griseus
3.4.11.24 8
-
 assay at Streptomyces griseus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.24 Streptomyces griseus isoelectric focusing
-
 5.2
3.4.11.24 Streptomyces griseus recombinant enzyme, isoelectric focusing and amino acid sequence calculation
-
 5.2