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Literature summary extracted from

  • Yao, Y.N.; Wang, L.; Wu, X.F.; Wang, E.D.
    Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli (2003), Protein Expr. Purif., 30, 112-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 the mitochondrial isozyme is cloned and expressed in Escherichia coli with the same N-terminus as that processed in the mitochondria of insect cells as His-tagged protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.01
-
 L-leucine recombinant mitochondrial isozyme mutant, 37°C Homo sapiens
6.1.1.4 0.021
-
 L-leucine recombinant mitochondrial isozyme mutant, 37°C Homo sapiens
6.1.1.4 0.99
-
 ATP recombinant mitochondrial isozyme mutant, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Homo sapiens
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.1.1.4 proteolytic modification processing of the mitochondrial isozyme by removing of the mitochondrial targeting presequence in the mitochondria of insect cells Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant N-terminally mutated His-tagged mitochondrial isozyme Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 0.011
-
 recombinant mitochondrial isozyme mutant in cell lysate Homo sapiens
6.1.1.4 0.128
-
 purified recombinant mitochondrial isozyme mutant Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information activity with mitochondrial tRNA mutants associated with some human mitochondrion-related neuromuscular disorders Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucine translase
-
 Homo sapiens
6.1.1.4 Leucine--tRNA ligase
-
 Homo sapiens
6.1.1.4 Leucyl-transfer ribonucleate synthetase
-
 Homo sapiens
6.1.1.4 Leucyl-transfer ribonucleic acid synthetase
-
 Homo sapiens
6.1.1.4 Leucyl-transfer RNA synthetase
-
 Homo sapiens
6.1.1.4 Leucyl-tRNA synthetase
-
 Homo sapiens
6.1.1.4 LeuRS
-
 Homo sapiens
6.1.1.4 Synthetase, leucyl-transfer ribonucleate
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.18
-
 L-leucine recombinant mitochondrial isozyme mutant, 37°C Homo sapiens
6.1.1.4 0.22
-
 ATP recombinant mitochondrial isozyme mutant, 37°C Homo sapiens
6.1.1.4 0.23
-
 L-leucine recombinant mitochondrial isozyme mutant, 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
 Homo sapiens