BRENDA - Enzyme Database show

Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein

Tsang, E.W.; Hu, Z.; Chang, Q.; McGregor, D.I.; Keller, W.A.; Protein Expr. Purif. 29, 193-201 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.3.8
glutamate
3fold activation of tagless recombinant enzyme
Brassica napus
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Brassica napus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
45500
-
2 * 45500, SDS-PAGE, native and recombinant GSA-AT
Brassica napus
5.4.3.8
88000
-
native and recombinant GSA-AT, gel filtration
Brassica napus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-amino-5-oxopentanoate
Brassica napus
involved in chlorophyll biosynthesis
5-aminolevulinate
-
Brassica napus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Brassica napus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.3.8
recombinant GSA-AT
Brassica napus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.015
-
recombinant GSA-AT
Brassica napus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-amino-5-oxopentanoate
-
653764
Brassica napus
5-aminolevulinate
-
653764
Brassica napus
r
5.4.3.8
(S)-4-amino-5-oxopentanoate
involved in chlorophyll biosynthesis
653764
Brassica napus
5-aminolevulinate
-
653764
Brassica napus
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.3.8
dimer
2 * 45500, SDS-PAGE, native and recombinant GSA-AT
Brassica napus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
-
Brassica napus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.3.8
glutamate
3fold activation of tagless recombinant enzyme
Brassica napus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
expression in Escherichia coli
Brassica napus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
-
Brassica napus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
45500
-
2 * 45500, SDS-PAGE, native and recombinant GSA-AT
Brassica napus
5.4.3.8
88000
-
native and recombinant GSA-AT, gel filtration
Brassica napus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-amino-5-oxopentanoate
Brassica napus
involved in chlorophyll biosynthesis
5-aminolevulinate
-
Brassica napus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
recombinant GSA-AT
Brassica napus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.015
-
recombinant GSA-AT
Brassica napus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-amino-5-oxopentanoate
-
653764
Brassica napus
5-aminolevulinate
-
653764
Brassica napus
r
5.4.3.8
(S)-4-amino-5-oxopentanoate
involved in chlorophyll biosynthesis
653764
Brassica napus
5-aminolevulinate
-
653764
Brassica napus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.3.8
dimer
2 * 45500, SDS-PAGE, native and recombinant GSA-AT
Brassica napus