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Literature summary extracted from

  • Dando, P.M.; Fortunato, M.; Strand, G.B.; Smith, T.S.; Barrett, A.J.
    Pyroglutamyl-peptidase I: cloning, sequencing, and characterisation of the recombinant human enzyme (2003), Protein Expr. Purif., 28, 111-119.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.19.3 thio-reducing agent absolute requirement Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 expressed by use of a baculovirus vector in Spodoptera frugiperda Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 2-Pyrrolidone reversible Homo sapiens
3.4.19.3 Ca2+ 1 mM, 20% inhibition Homo sapiens
3.4.19.3 Cu2+ 1 mM, 96% inhibition Homo sapiens
3.4.19.3 E64 0.1 mM, 15% inhibition Homo sapiens
3.4.19.3 NEM reversible Homo sapiens
3.4.19.3 Ni2+ 0.1 mM, 98% inhibition Homo sapiens
3.4.19.3 Zn2+ 1 mM, 98% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.3 0.05
-
 pyroglutamyl-4-methylcoumaryl-7-amide pH 7.8, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 23188
-
 1 * 23188, calculation from nucleotide sequence Homo sapiens
3.4.19.3 24000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Homo sapiens Q9NXJ5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.3 colon mucosa Homo sapiens
-
3.4.19.3 mucosa from colon Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.19.3 -20°C, retains more than 90% of its activity for 1 month in the presence of a thiol-reducing agent Homo sapiens
3.4.19.3 4°C, pH 7.8, stable for at least 1 week Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 ACE inhibitor + H2O contains the N-terminal dipeptide pyroglutamyl-Trp- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 bombesin + H2O contains the N-terminal dipeptide pyroglutamyl-Arg- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 bradykinin potentiator B + H2O contains the N-terminal dipeptide pyroglutamyl-Gly- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 leukopyrokinin + H2O contains the N-terminal dipeptide pyroglutamyl-Thr- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 luteinizing hormone releasing hormone + H2O contains the N-terminal dipeptide pyroglutamyl-His- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 neurotensin + H2O contains the N-terminal dipeptide pyroglutamyl-Leu- Homo sapiens pyroglutamate + ?
-
 ?
3.4.19.3 pyroglutamyl-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens pyroglutamate + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.3 monomer 1 * 23188, calculation from nucleotide sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.19.3 PGPEP1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.3 50
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 40
-
 4.5 h, stable up to Homo sapiens
3.4.19.3 50
-
 90 min, enzyme is completely stable in crude preparation Homo sapiens
3.4.19.3 60
-
 30 min, activity is almost completely lost Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7 8.5 hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.19.3 6 9 pH 6.0: about 75% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.19.3 0.03
-
 NEM pH 7.8, 37°C Homo sapiens
3.4.19.3 0.05
-
 2-Pyrrolidone pH 7.8, 37°C Homo sapiens