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Literature summary extracted from

  • Ubeidat, M.; Rutherford, C.L.
    Purification and renaturation of Dictyostelium recombinant alkaline phosphatase by continuous elution electrophoresis (2003), Protein Expr. Purif., 27, 375-383.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 94% fragment of gene alp cDNA, functional overexpression in Escherichia coli BL21(DE3) as internally His-tagged enzyme use of continous electrophoresis procedure Dictyostelium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 80000
-
 x * 80000, recombinant enzyme, SDS-PAGE Dictyostelium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Dictyostelium sp.
-
 gene alp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 recombinant enzyme in nclusion bodies after expression in Escherichia coli Dictyostelium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Dictyostelium sp.
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.1 recombinant His-tagged enzyme from inclusion bodies after expression in Escherichia coli, solubilization by 1% SDS in ddH2O from purified inclusion bodies Dictyostelium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 0.08
-
 purified enzyme, substrate cAMP Dictyostelium sp.
3.1.3.1 0.33
-
 purified enzyme, substrate ATP Dictyostelium sp.
3.1.3.1 0.65
-
 purified enzyme, substrate beta-glycerol phosphate Dictyostelium sp.
3.1.3.1 0.7
-
 purified enzyme, substrates 4-nitrophenyl phosphate and 5'-AMP Dictyostelium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Dictyostelium sp. 4-nitrophenol + phosphate
-
 ?
3.1.3.1 5'-AMP + H2O
-
 Dictyostelium sp. adenosine + phosphate
-
 ?
3.1.3.1 ATP + H2O
-
 Dictyostelium sp. ADP + phosphate
-
 ?
3.1.3.1 beta-glycerol phosphate + H2O
-
 Dictyostelium sp. glycerol + phosphate
-
 ?
3.1.3.1 cAMP + H2O
-
 Dictyostelium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 80000, recombinant enzyme, SDS-PAGE Dictyostelium sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Dictyostelium sp.
3.1.3.1 ALP
-
 Dictyostelium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 25
-
 assay at Dictyostelium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9
-
 approximately Dictyostelium sp.