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Literature summary extracted from

  • Jayalakshmi, R.; Sumathy, K.; Balaram, H.
    Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli (2002), Protein Expr. Purif., 25, 65-72.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.4 medicine most parasitic protozoa lack the de novo purine biosynthetic pathway and rely exclusively on the salvage pathway for their purine biosynthetic pathway, enzymes of the salvage pathway are candidate drug targets Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4 hyperexpressed in Escherichia coli BL21 (DE3), complementation of purA mutant strain H1238 Plasmodium falciparum

General Stability

EC Number General Stability Organism
6.3.4.4 requires presence of dithiothreitol during the entire course of purification for activity Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.0048
-
 GTP pH 7.5, 37°C Plasmodium falciparum
6.3.4.4 0.0228
-
 IMP pH 7.5, 37°C Plasmodium falciparum
6.3.4.4 1.4
-
 aspartate pH 7.5, 37°C Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 50000
-
 2 * 50000, recombinant protein, SDS-PAGE Plasmodium falciparum
6.3.4.4 105000
-
 gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate Plasmodium falciparum salvage pathway GDP + phosphate + adenylosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4 recombinant protein Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.4 1144
-
-
 Plasmodium falciparum

Storage Stability

EC Number Storage Stability Organism
6.3.4.4 4°C, glycerol and EDTA stabilizes enzyme activity during storage, stable for at least 3 weeks Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate
-
 Plasmodium falciparum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate salvage pathway Plasmodium falciparum GDP + phosphate + adenylosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.4 dimer 2 * 50000, recombinant protein, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 Adenylosuccinate synthetase
-
 Plasmodium falciparum
6.3.4.4 AdSS
-
 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 7.5
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.4 5.8 8.8
-
 Plasmodium falciparum