Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, S.; Albright, C.F.; Fish, B.H.; George, H.J.; Selling, B.H.; Hollis, G.F.; Wynn, R.
    Expression, purification, and kinetic characterization of full-length human fibroblast activation protein (2002), Protein Expr. Purif., 24, 274-281.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B28 2-mercaptoethanol inhibition at 140 mM Homo sapiens
3.4.21.B28 chymostatin 50% inhibition at 0.16 mM Homo sapiens
3.4.21.B28 dimethyl sulfoxide 50% inhibition at 4% Homo sapiens
3.4.21.B28 dithiothreitol inhibition at 10 mM Homo sapiens
3.4.21.B28 phenylmethylsulfonyl fluoride more than 50% inhibition at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B28 0.2
-
 Ala-Pro-4-trifluoromethylcoumarin-7-amide pH 8.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B28 Homo sapiens Q12884
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B28 glycoprotein two isoforms: glycosylated and nonglycosylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B28 glycosylated form, near homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B28 Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O
-
 Homo sapiens Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.21.B28 additional information glycosylated form has both postprolyl depeptidyl peptidase and postgelatinase activity, nonglycosylated isoform has no detectable gelatinase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B28 FAP
-
 Homo sapiens
3.4.21.B28 fibroblast activation factor
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B28 2
-
 Ala-Pro-4-trifluoromethylcoumarin-7-amide pH 8.5 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B28 8.5 9
-
 Homo sapiens