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Literature summary extracted from

  • Savopoulos, J.W.; Hibbs, M.; Jones, E.J.; Mensah, L.; Richardson, C.; Fosberry, A.; Downes, R.; Fox, S.G.; Brown, J.R.; Jenkins, O.
    Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus (2001), Protein Expr. Purif., 21, 470-484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.20 genes pheS and pheT encoding the alpha and beta subunits in one transcriptional operon, DNA and amino acid sequence determination and analysis, overexpression of both genes in an artificial operon in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.00094
-
 L-phenylalanine recombinant enzyme, pH 7.9, 22°C Staphylococcus aureus
6.1.1.20 0.0012
-
 L-phenylalanine native enzyme, pH 7.9, 22°C Staphylococcus aureus
6.1.1.20 0.122
-
 ATP recombinant enzyme, pH 7.9, 22°C Staphylococcus aureus
6.1.1.20 0.147
-
 ATP native enzyme, pH 7.9, 22°C Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 40000
-
 alpha-subunit of recombinant enzyme, mass spectroscopy Staphylococcus aureus
6.1.1.20 89090
-
 beta-subunit of recombinant enzyme, mass spectroscopy Staphylococcus aureus
6.1.1.20 90000
-
 2 * 40000, alpha subunit, + 2 * 90000, beta-subunit, (alphabeta)2, recombinant enzyme, SDS-PAGE Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Staphylococcus aureus
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Staphylococcus aureus
-
 strain WCUH29 and Oxford strain, genes pheS and pheT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20 native enzyme from Oxford strain, 50% purity, recombinant enzyme overexpressed in Escherichia coli, over 90% purity Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.20 0.074
-
 purified native enzyme Staphylococcus aureus
6.1.1.20 0.12
-
 purified recombinant enzyme Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Staphylococcus aureus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 tetramer 2 * 40000, alpha subunit, + 2 * 90000, beta-subunit, (alphabeta)2, recombinant enzyme, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 CML33
-
 Staphylococcus aureus
6.1.1.20 FRS
-
 Staphylococcus aureus
6.1.1.20 HSPC173
-
 Staphylococcus aureus
6.1.1.20 L-Phenylalanyl-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanine translase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanine--tRNA ligase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl transfer ribonucleic acid synthetase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl-transfer ribonucleate synthetase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl-transfer RNA ligase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl-transfer RNA synthetase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl-tRNA ligase
-
 Staphylococcus aureus
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.20 PheRS
-
 Staphylococcus aureus
6.1.1.20 Synthetase, phenylalanyl-transfer ribonucleate
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 22
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 7.9
-
 assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP a 15fold excess is required for optimal activity Staphylococcus aureus