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Literature summary extracted from

  • Ho, C.K.; Shuman, S.
    Bacteriophage T4 RNA ligase 2 (gp24.1) exemplifies a family of RNA ligases found in all phylogenetic domains (2002), Proc. Natl. Acad. Sci. USA, 99, 12709-12714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3 transformed into Escherichia coli BL21(DE3) Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.3 E204A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
6.5.1.3 H37A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
6.5.1.3 H37D substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
6.5.1.3 K225A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
6.5.1.3 K227A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
6.5.1.3 K35A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.3 42000
-
 recombinant protein, adsorption to Ni-agarose and step-elution with imidazole Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4 participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 recombinant enzyme Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 gp24.1
-
 Tequatrovirus T4
6.5.1.3 Rnl2
-
 Tequatrovirus T4
6.5.1.3 T4 RNA ligase 2
-
 Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.3 5.5 7
-
 Tequatrovirus T4
6.5.1.3 6.5
-
 adenylyltransferase activity Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.3 5.5 8 adenylyltransferase activity Tequatrovirus T4