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Literature summary extracted from

  • Shimada, A.; Nureki, O.; Goto, M.; Takahashi, S.; Yokoyama, S.
    Structural and mutational studies of the recognition of the arginine tRNA-specific major identity element, A20, by arginyl-tRNA synthetase (2001), Proc. Natl. Acad. Sci. USA, 98, 13537-13542.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.19 overexpression in Escherichia coli of wild type and several mutant enzymes Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.19 crystal structure solved by multiple isomorphous replacement Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.19 N79D can use tRNAArg with G20 as substrate Thermus thermophilus
6.1.1.19 N79E can use tRNAArg with G20 or U20 as substrate Thermus thermophilus
6.1.1.19 N79K can use tRNAArg with G20 as substrate Thermus thermophilus
6.1.1.19 N79Q can use tRNAArg with G20 as substrate Thermus thermophilus
6.1.1.19 N79R can use tRNAArg with G20 or U20 as substrate Thermus thermophilus
6.1.1.19 Y77A inactive mutant Thermus thermophilus
6.1.1.19 Y77F slight effect on activity Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 additional information
-
 additional information Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20 Thermus thermophilus
6.1.1.19 0.0098
-
 tRNAArg adenine at position 20 of the tRNAArg is required for activity, pH 7.5, 65°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Thermus thermophilus Q93RP5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Thermus thermophilus AMP + diphosphate + L-arginyl-tRNAArg
-
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