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Literature summary extracted from

  • Bar-Peled, M.; Griffith, C.L.; Doering, T.L.
    Functional cloning and characterization of a UDP-glucuronic acid decarboxylase: the pathogenic fungus Cryptococcus neoformans elucidates UDP-xylose synthesis (2001), Proc. Natl. Acad. Sci. USA, 98, 12003-12008.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.35 gene UXS1 cloned and expressed in Escherichia coli BL21 (DE3)pLysS Cryptococcus neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.35 CaCl2 slight inhibition Cryptococcus neoformans
4.1.1.35 MgCl2 slight inhibition Cryptococcus neoformans
4.1.1.35 MnCl2 slight inhibition Cryptococcus neoformans
4.1.1.35 NADH not affected by NADP+, only slightly inhibited by NADPH Cryptococcus neoformans
4.1.1.35 UDP
-
 Cryptococcus neoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.35 additional information metal ions are not required Cryptococcus neoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.35 46500
-
 predicted from amino acid sequence Cryptococcus neoformans
4.1.1.35 47000
-
 recombinant enzyme, bacterial expressed Cryptococcus neoformans

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.35 Cryptococcus neoformans Q96V00 pathogenic fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.35
-
 Cryptococcus neoformans

Storage Stability

EC Number Storage Stability Organism
4.1.1.35 -70°C, stable for several months Cryptococcus neoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.35 UDP-D-glucuronate
-
 Cryptococcus neoformans UDP-D-xylose + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.35 UDP-glucuronic acid decarboxylase
-
 Cryptococcus neoformans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.35 37
-
-
 Cryptococcus neoformans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.35 23 42
-
 Cryptococcus neoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.35 7.5
-
-
 Cryptococcus neoformans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.35 6.5 8.5
-
 Cryptococcus neoformans