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Literature summary extracted from

  • Soell, D.; Becker, H.D.; Plateau, P.; Blanquet, S.; Ibba, M.
    Context-dependent anticodon recognition by class I lysyl-tRNA synthetases (2000), Proc. Natl. Acad. Sci. USA, 97, 14224-14228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.6 overexpresssion of His-tagged enzyme in Escherichia coli Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.6 0.00033
-
 tRNALysGUU
-
 Borreliella burgdorferi
6.1.1.6 0.0004
-
 tRNALysUUU
-
 Borreliella burgdorferi
6.1.1.6 0.00056
-
 tRNALysCUU
-
 Borreliella burgdorferi
6.1.1.6 0.0015
-
 tRNALysUUUmodified
-
 Borreliella burgdorferi
6.1.1.6 0.0018
-
 tRNALysUGU
-
 Borreliella burgdorferi
6.1.1.6 0.0028
-
 tRNALysUUC
-
 Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys Helicobacter pylori
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys Methanococcus maripaludis
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys Borreliella burgdorferi
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 additional information Helicobacter pylori phylogenetic comparison ?
-
 ?
6.1.1.6 additional information Methanococcus maripaludis phylogenetic comparison ?
-
 ?
6.1.1.6 additional information Borreliella burgdorferi phylogenetic comparison ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Borreliella burgdorferi
-
 recombinant purified bacterial class I enzyme
-
6.1.1.6 Helicobacter pylori
-
 gene lysS, class II enzyme
-
6.1.1.6 Methanococcus maripaludis
-
 recombinant purified archaeal class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.6 recombinant from overexpressing Escherichia coli Helicobacter pylori

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.6 ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys bases N73, U35 and U36 play an important role in tRNA substrate recognition Borreliella burgdorferi
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.6 additional information
-
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys
-
 Helicobacter pylori AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys
-
 Methanococcus maripaludis AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys
-
 Borreliella burgdorferi AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys tRNA substrate from Escherichia coli, bases N73, and U36 play an important role in tRNA substrate recognition Methanococcus maripaludis AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys tRNA substrate from Escherichia coli, bases U35 and U36 play an important role in tRNA substrate recognition Helicobacter pylori AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysCUU anticodon variant tRNA substrate from Escherichia coli Borreliella burgdorferi AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysGUU anticodon variant tRNA substrate from Escherichia coli Borreliella burgdorferi AMP + L-lysyl-tRNALysGUU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUGU anticodon variant tRNA substrate from Escherichia coli Borreliella burgdorferi AMP + L-lysyl-tRNALysUGU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUC anticodon variant tRNA substrate from Escherichia coli Borreliella burgdorferi AMP + L-lysyl-tRNALysUUC + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUU anticodon variant tRNA substrate from Escherichia coli Borreliella burgdorferi AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUUmodified anticodon variant tRNA substrate from Escherichia coli, t6 modification of base A37 Borreliella burgdorferi AMP + L-lysyl-tRNALysUUUmodified + diphosphate
-
 ?
6.1.1.6 additional information phylogenetic comparison Helicobacter pylori ?
-
 ?
6.1.1.6 additional information phylogenetic comparison Methanococcus maripaludis ?
-
 ?
6.1.1.6 additional information phylogenetic comparison Borreliella burgdorferi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.6 L-Lysine-transfer RNA ligase
-
 Helicobacter pylori
6.1.1.6 L-Lysine-transfer RNA ligase
-
 Methanococcus maripaludis
6.1.1.6 L-Lysine-transfer RNA ligase
-
 Borreliella burgdorferi
6.1.1.6 Lysine translase
-
 Helicobacter pylori
6.1.1.6 Lysine translase
-
 Methanococcus maripaludis
6.1.1.6 Lysine translase
-
 Borreliella burgdorferi
6.1.1.6 Lysine--tRNA ligase
-
 Helicobacter pylori
6.1.1.6 Lysine--tRNA ligase
-
 Methanococcus maripaludis
6.1.1.6 Lysine--tRNA ligase
-
 Borreliella burgdorferi
6.1.1.6 Lysine-tRNA synthetase
-
 Helicobacter pylori
6.1.1.6 Lysine-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.6 Lysine-tRNA synthetase
-
 Borreliella burgdorferi
6.1.1.6 LysRS
-
 Helicobacter pylori
6.1.1.6 LysRS
-
 Methanococcus maripaludis
6.1.1.6 LysRS
-
 Borreliella burgdorferi
6.1.1.6 Lysyl-transfer ribonucleate synthetase
-
 Helicobacter pylori
6.1.1.6 Lysyl-transfer ribonucleate synthetase
-
 Methanococcus maripaludis
6.1.1.6 Lysyl-transfer ribonucleate synthetase
-
 Borreliella burgdorferi
6.1.1.6 Lysyl-transfer RNA synthetase
-
 Helicobacter pylori
6.1.1.6 Lysyl-transfer RNA synthetase
-
 Methanococcus maripaludis
6.1.1.6 Lysyl-transfer RNA synthetase
-
 Borreliella burgdorferi
6.1.1.6 Lysyl-tRNA synthetase
-
 Helicobacter pylori
6.1.1.6 Lysyl-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.6 Lysyl-tRNA synthetase
-
 Borreliella burgdorferi
6.1.1.6 Synthetase, lysyl-transfer ribonucleate
-
 Helicobacter pylori
6.1.1.6 Synthetase, lysyl-transfer ribonucleate
-
 Methanococcus maripaludis
6.1.1.6 Synthetase, lysyl-transfer ribonucleate
-
 Borreliella burgdorferi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.6 0.00018
-
 tRNALysUUC
-
 Borreliella burgdorferi
6.1.1.6 0.0004
-
 tRNALysUGU
-
 Borreliella burgdorferi
6.1.1.6 0.0095
-
 tRNALysUUU
-
 Borreliella burgdorferi
6.1.1.6 0.0095
-
 tRNALysGUU
-
 Borreliella burgdorferi
6.1.1.6 0.014
-
 tRNALysCUU
-
 Borreliella burgdorferi
6.1.1.6 0.12
-
 tRNALysUUUmodified
-
 Borreliella burgdorferi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.6 ATP
-
 Helicobacter pylori
6.1.1.6 ATP
-
 Methanococcus maripaludis
6.1.1.6 ATP
-
 Borreliella burgdorferi