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Literature summary extracted from

  • Lunn, J.E.; Ashton, A.R.; Hatch, M.D.; Heldt, H.W.
    Purification, molecular cloning, and sequence analysis of sucrose-6F-phosphate phosphohydrolase from plants (2000), Proc. Natl. Acad. Sci. USA, 97, 12914-12919.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.24 expression in Escherichia coli Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.24 EDTA
-
 Oryza sativa
3.1.3.24 sucrose weak competitive inhibition Oryza sativa
3.1.3.24 Zn2+
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.24 0.065
-
 sucrose 6-phosphate pH 7.0, 30°C Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.24 Mg2+ highly dependent on, can partially be replaced by Mn2+ Oryza sativa
3.1.3.24 Mn2+ can partially replace Mg2+, 37% of the activity with Mg2+ at 0.1 mM Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.24 47213
-
 x * 47213, DNA sequence calculation Zea mays
3.1.3.24 50000
-
 2 * 50000, SDS-PAGE Oryza sativa
3.1.3.24 100000
-
 gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.24 sucrose 6-phosphate + H2O Oryza sativa final step of sucrose biosynthesis sucrose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.24 Oryza sativa
-
-
-
3.1.3.24 Zea mays Q9FQ11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.24 25000fold, to homogeneity Oryza sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.24 sucrose 6F-phosphate + H2O = sucrose + phosphate N-terminal catalytic domain Zea mays
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.24 leaf
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.24 0.37
-
 recombinant enzyme in extract of expressing Escherichia coli Zea mays
3.1.3.24 1250
-
 purified enzyme Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.24 additional information no activity with fructose 6-phosphate Oryza sativa ?
-
 ?
3.1.3.24 sucrose 6-phosphate + H2O
-
 Zea mays sucrose + phosphate
-
 ?
3.1.3.24 sucrose 6-phosphate + H2O highly specific for Oryza sativa sucrose + phosphate
-
 ?
3.1.3.24 sucrose 6-phosphate + H2O final step of sucrose biosynthesis Oryza sativa sucrose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.24 ? x * 47213, DNA sequence calculation Zea mays
3.1.3.24 dimer 2 * 50000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.24 SPP
-
 Oryza sativa
3.1.3.24 SPP
-
 Zea mays