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Literature summary extracted from

  • Martin, E.; Sharina, I.; Kots, A.; Murad, F.
    A constitutively activated mutant of human soluble guanylyl cyclase (sGC): Implication for the mechanism of sGC activation (2003), Proc. Natl. Acad. Sci. USA, 100, 9208-9213.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.2 NO
-
 Homo sapiens
4.6.1.2 sodium nitroprusside NO donor Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.2 expression of sGCalpha and sGCbeta subunit in Sf1 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.2 H105C heme-deficient mutant, 70 times higher basal sGC activity than wild-type, basal activity is not affected by NO, heme reconstituted mutant regains No activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.2 additional information heme moiety of sGC has some inhibitory function on sGC activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.2 soluble
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.2 GTP Homo sapiens
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.2 recombinant sGC Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.2 22.1
-
 NO-stimulated sGC Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Homo sapiens 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.2 dimer alpha1beta1, heme moiety is localized in beta1 subunit Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 sGC
-
 Homo sapiens
4.6.1.2 soluble guanylyl cyclase
-
 Homo sapiens