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Literature summary extracted from

  • Zhou, R.; Cheng, L.; Wayne, R.
    Purification and characterization of sorbitol-6-phosphate phosphatase from apple leaves (2003), Plant Sci., 165, 227-232.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.50 fluoride
-
Malus domestica
3.1.3.50 molybdate
-
Malus domestica
3.1.3.50 phosphate
-
Malus domestica
3.1.3.50 sorbitol competitive Malus domestica
3.1.3.50 vanadate
-
Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.50 0.85
-
D-sorbitol-6-phosphate pH 6.8, 30°C Malus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.50 Mg2+ required Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.50 61000
-
gel filtration Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.50 Malus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.50
-
Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.50 leaf
-
Malus domestica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.50 89.8
-
pH 6.8, 30°C Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.50 D-sorbitol 6-phosphate + H2O highly specific Malus domestica D-sorbitol + phosphate
-
?
3.1.3.50 additional information no substrate: other phosphate esters than sorbitol-6-phosphate Malus domestica ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.50 monomer 1 * 61000, SDS-PAGE Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.50 6.8
-
-
Malus domestica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.50 109
-
D-sorbitol pH 6.8, 30°C Malus domestica