BRENDA - Enzyme Database

Cloning and characterisation of S-formylglutathione hydrolase from Arabidopsis thaliana: a pathway for formaldehyde detoxification

Haslam, R.; Rust, S.; Pallett, K.; Cole, D.; Coleman, J.; Plant Physiol. Biochem. 40, 281-288 (2002)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.12
expression in Escherichia coli
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
DTNB
48% inhibition at 0.001 mM
Arabidopsis thaliana
3.1.2.12
p-hydroxymercuribenzoate
59% inhibition at 0.001 mM
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.087
-
4-yethylumbelliferyl acetate
pH 7.0, 25°C
Arabidopsis thaliana
3.1.2.12
0.318
-
S-formylglutathione
pH 7.0, 25°C
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
x * 30000, about, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Arabidopsis thaliana
key enzyme of the formaldehyde detoxification, glutathione recycling
glutathione + formate
-
Arabidopsis thaliana
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Arabidopsis thaliana
-
ecotype Columbia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
4.86
-
recombinant enzyme, crude enzyme extract
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
-
653549
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
653549
Arabidopsis thaliana
?
3.1.2.12
4-nitrophenyl acetate + H2O
-
653549
Arabidopsis thaliana
4-nitrophenol + formate
-
653549
Arabidopsis thaliana
?
3.1.2.12
additional information
2-nitrophenyl acetate is a poor substrate
653549
Arabidopsis thaliana
?
-
653549
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
-
653549
Arabidopsis thaliana
glutathione + formate
-
653549
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
key enzyme of the formaldehyde detoxification, glutathione recycling
653549
Arabidopsis thaliana
glutathione + formate
-
653549
Arabidopsis thaliana
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
?
x * 30000, about, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
25
-
assay at
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
expression in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
DTNB
48% inhibition at 0.001 mM
Arabidopsis thaliana
3.1.2.12
p-hydroxymercuribenzoate
59% inhibition at 0.001 mM
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.087
-
4-yethylumbelliferyl acetate
pH 7.0, 25°C
Arabidopsis thaliana
3.1.2.12
0.318
-
S-formylglutathione
pH 7.0, 25°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
x * 30000, about, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Arabidopsis thaliana
key enzyme of the formaldehyde detoxification, glutathione recycling
glutathione + formate
-
Arabidopsis thaliana
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
4.86
-
recombinant enzyme, crude enzyme extract
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
-
653549
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
653549
Arabidopsis thaliana
?
3.1.2.12
4-nitrophenyl acetate + H2O
-
653549
Arabidopsis thaliana
4-nitrophenol + formate
-
653549
Arabidopsis thaliana
?
3.1.2.12
additional information
2-nitrophenyl acetate is a poor substrate
653549
Arabidopsis thaliana
?
-
653549
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
-
653549
Arabidopsis thaliana
glutathione + formate
-
653549
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
key enzyme of the formaldehyde detoxification, glutathione recycling
653549
Arabidopsis thaliana
glutathione + formate
-
653549
Arabidopsis thaliana
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
?
x * 30000, about, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
25
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7
-
assay at
Arabidopsis thaliana