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Literature summary extracted from

  • Haslam, R.; Rust, S.; Pallett, K.; Cole, D.; Coleman, J.
    Cloning and characterisation of S-formylglutathione hydrolase from Arabidopsis thaliana: a pathway for formaldehyde detoxification (2002), Plant Physiol. Biochem., 40, 281-288.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.12 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.12 DTNB 48% inhibition at 0.001 mM Arabidopsis thaliana
3.1.2.12 p-hydroxymercuribenzoate 59% inhibition at 0.001 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.12 0.087
-
 4-yethylumbelliferyl acetate pH 7.0, 25°C Arabidopsis thaliana
3.1.2.12 0.318
-
 S-formylglutathione pH 7.0, 25°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.12 30000
-
 x * 30000, about, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.12 S-formylglutathione + H2O Arabidopsis thaliana key enzyme of the formaldehyde detoxification, glutathione recycling glutathione + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 Arabidopsis thaliana
-
 ecotype Columbia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.12 4.86
-
 recombinant enzyme, crude enzyme extract Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 4-methylumbelliferyl acetate + H2O
-
 Arabidopsis thaliana 4-methylumbelliferol + acetate
-
 ?
3.1.2.12 4-nitrophenyl acetate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + acetate
-
 ?
3.1.2.12 additional information 2-nitrophenyl acetate is a poor substrate Arabidopsis thaliana ?
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Arabidopsis thaliana glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O key enzyme of the formaldehyde detoxification, glutathione recycling Arabidopsis thaliana glutathione + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.12 ? x * 30000, about, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.2.12 FGH
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.12 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.12 7
-
 assay at Arabidopsis thaliana