BRENDA - Enzyme Database

4-Coumarate:coenzyme A ligase has the catalytic capacity to synthesize and reuse various (di)adenosine polyphosphates

Pietrowska-Borek, M.; Stuible, H.P.; Kombrink, E.; Guranowski, A.; Plant Physiol. 131, 1401-1410 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.2.1.12
-
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.2.1.12
M293P/K320L
strongly affects Km for all substrates, capable in using ferulate, capable in adenosine 5'-tetraphosphate formation at 10fold higher rate than wild type enzyme
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.12
0.004
-
ATP
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.024
-
caffeate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.027
-
4-coumarate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.046
-
ferulate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.048
-
caffeate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.064
-
adenosine 5'-tetraphosphate
reverse reaction, M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.233
-
4-coumarate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.292
-
cinnamate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
6.35
-
cinnamate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.12
Co2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
Mg2+
required
Arabidopsis thaliana
6.2.1.12
Mn2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
additional information
no effect with Ca2+
Arabidopsis thaliana
6.2.1.12
Ni2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
Zn2+
can partially substitute for Mg2+, very low activity
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.12
ATP + 4-coumarate + CoA
Arabidopsis thaliana
plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis
AMP + diphosphate + 4-coumaroyl-CoA
-
Arabidopsis thaliana
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.12
Arabidopsis thaliana
-
isoform 4CL2
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.12
recombinant enzyme
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.12
4-coumarate + MgATP2- + tetrapolyphosphate
CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
adenosine 5'-pentaphosphate + ?
-
653536
Arabidopsis thaliana
r
6.2.1.12
4-coumarate + MgATP2- + tripolyphosphate
CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
adenosine 5'-tetraphosphate + ?
-
653536
Arabidopsis thaliana
r
6.2.1.12
ATP + 4-coumarate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + 4-coumaroyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + 4-coumarate + CoA
plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis
653536
Arabidopsis thaliana
AMP + diphosphate + 4-coumaroyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + caffeate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + caffeoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + cinnamate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + cinnamoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + ferulate + CoA
M293P/K320L mutant enzyme, no substrate for wild type enzyme
653536
Arabidopsis thaliana
AMP + diphosphate + feruloyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + sinapate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + sinapoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
MgATP2-
no additional substrate, CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
diadenosine 5',5'',P1,P4-tetraphosphate + ?
-
653536
Arabidopsis thaliana
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.12
6
8
adenosine 5' tetraphosphate formation
Arabidopsis thaliana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.2.1.12
4.5
9
half maximal activity, adenosine 5' tetraphosphate foormation
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.12
-
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.2.1.12
M293P/K320L
strongly affects Km for all substrates, capable in using ferulate, capable in adenosine 5'-tetraphosphate formation at 10fold higher rate than wild type enzyme
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.12
0.004
-
ATP
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.024
-
caffeate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.027
-
4-coumarate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.046
-
ferulate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.048
-
caffeate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.064
-
adenosine 5'-tetraphosphate
reverse reaction, M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.233
-
4-coumarate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
0.292
-
cinnamate
M293P/K320L mutant enzyme, pH 7.0, 30°C
Arabidopsis thaliana
6.2.1.12
6.35
-
cinnamate
wild-type enzyme, pH 7.0, 30°C
Arabidopsis thaliana
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.12
Co2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
Mg2+
required
Arabidopsis thaliana
6.2.1.12
Mn2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
additional information
no effect with Ca2+
Arabidopsis thaliana
6.2.1.12
Ni2+
can partially substitute for Mg2+
Arabidopsis thaliana
6.2.1.12
Zn2+
can partially substitute for Mg2+, very low activity
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.12
ATP + 4-coumarate + CoA
Arabidopsis thaliana
plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis
AMP + diphosphate + 4-coumaroyl-CoA
-
Arabidopsis thaliana
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.12
recombinant enzyme
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.12
4-coumarate + MgATP2- + tetrapolyphosphate
CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
adenosine 5'-pentaphosphate + ?
-
653536
Arabidopsis thaliana
r
6.2.1.12
4-coumarate + MgATP2- + tripolyphosphate
CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
adenosine 5'-tetraphosphate + ?
-
653536
Arabidopsis thaliana
r
6.2.1.12
ATP + 4-coumarate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + 4-coumaroyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + 4-coumarate + CoA
plant phenylpropanoid metabolism, 4CL2 involved in coenzyme A ester formation and cocatalytic AMP-donor in (di)adenosine polyphosphate synthesis
653536
Arabidopsis thaliana
AMP + diphosphate + 4-coumaroyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + caffeate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + caffeoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + cinnamate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + cinnamoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + ferulate + CoA
M293P/K320L mutant enzyme, no substrate for wild type enzyme
653536
Arabidopsis thaliana
AMP + diphosphate + feruloyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
ATP + sinapate + CoA
-
653536
Arabidopsis thaliana
AMP + diphosphate + sinapoyl-CoA
-
653536
Arabidopsis thaliana
?
6.2.1.12
MgATP2-
no additional substrate, CoA omitted from the incubation mixture
653536
Arabidopsis thaliana
diadenosine 5',5'',P1,P4-tetraphosphate + ?
-
653536
Arabidopsis thaliana
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.12
6
8
adenosine 5' tetraphosphate formation
Arabidopsis thaliana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.2.1.12
4.5
9
half maximal activity, adenosine 5' tetraphosphate foormation
Arabidopsis thaliana