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Literature summary extracted from

  • Bhardwaj, K.; Raju, A.; Rajasekharan, R.
    Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (phospho) lipase family (2001), Plant Physiol., 127, 1728-1738.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 potassium acetate 20-200 mM, 4-5fold increase in activity Oryza sativa
3.1.1.3 Sodium acetate 20 mM, 2fold increase in activity, no effect at higher concentration at pH 7.5 Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cd2+ about 70% inhibition at 2 mM Oryza sativa
3.1.1.3 CHAPS inhibitory at 0.5 mM Oryza sativa
3.1.1.3 Cu2+ about 70% inhibition at 2 mM Oryza sativa
3.1.1.3 Digitonin inhibitory at 0.5 mM Oryza sativa
3.1.1.3 diisopropyl fluorophosphate inhibits both lipase and phospholipase activities of the enzyme Oryza sativa
3.1.1.3 Mg2+ about 70% inhibition at 2 mM Oryza sativa
3.1.1.3 additional information no inhibition by Ca2+, Triton X-100 and NP-40 decreased the activity marginally at lower concentrations and then restorde the activity at 4 mM Oryza sativa
3.1.1.3 SDS 50% inhibition at 2mM Oryza sativa
3.1.1.3 Zn2+ about 70% inhibition at 2 mM Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 1.02
-
 phosphocholine pH 7.5, 37°C Oryza sativa
3.1.1.3 6.71
-
 triolein pH 7.5, 37°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 9400
-
 gel filtration and MALDI Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Oryza sativa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein
-
 Oryza sativa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 7.6fold to homogeneity Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 seed coat
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.00012
-
 purified enzyme, phospholipase activity Oryza sativa
3.1.1.3 0.38
-
 purified enzyme, lipase activity Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 diacylglycerol + H2O
-
 Oryza sativa fatty acid + acylglycerol
-
 ?
3.1.1.3 additional information enzyme also provides phospholipase A2 activity, it preferentially hydrolyzes the sn-2-position of the substrate Oryza sativa ?
-
 ?
3.1.1.3 phosphatidylethanolamine + H2O
-
 Oryza sativa ?
-
 ?
3.1.1.3 phosphocholine + H2O
-
 Oryza sativa ?
-
 ?
3.1.1.3 triolein + H2O enzyme shows no positional specificity towards the triacylglycerol Oryza sativa diolein + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 80
-
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 20 90 completely stable up to 40°C, 65% remaining activity at 60°C after 1 h, 76°C is the transition temperature, the enzyme is still active and retains most of its secondary structure at 90°C, 34% remaining activity at 90°C after 15 min pretreatment at 90°C, 16% remaining activity at 37°C after 15 min pretreatment at 90°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 11
-
-
 Oryza sativa