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Literature summary extracted from

  • Davy, A.; Thomsen, K.K.; Juliano, M.A.; Alves, L.C.; Svendsen, I.; Simpson, D.J.
    Purification and characterization of barley dipeptidyl peptidase IV (2000), Plant Physiol., 122, 425-432.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 4-(2-aminoethyl)benzenesulfonyl fluoride i.e. AEBSF Hordeum vulgare
3.4.14.5 Diprotin A specific inhibition Hordeum vulgare
3.4.14.5 Diprotin B specific inhibition Hordeum vulgare
3.4.14.5 additional information no inhibition by EDTA, E-64, pepstatin A or leupeptin Hordeum vulgare
3.4.14.5 PMSF
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 0.000071
-
 Lys-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00009
-
 Arg-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00034
-
 Ala-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00065
-
 Phe-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.0007
-
 Ile-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00073
-
 Tyr-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00093
-
 L-Thr-L-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00103
-
 His-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00109
-
 Leu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.0011
-
 Pro-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00126
-
 Ser-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00138
-
 Gln-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00217
-
 Met-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00298
-
 Glu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00324
-
 Asn-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00374
-
 Lys-Ala-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00688
-
 Gly-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 0.00888
-
 Asp-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 85000
-
 1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE Hordeum vulgare
3.4.14.5 105000
-
 gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Hordeum vulgare
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 glycoprotein the deglycosylated enzyme is still active Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5 from green barley malt, more than 43000fold Hordeum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.14.5 release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline serine protease Hordeum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 seed malt from green barley Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 additional information
-
 purified enzyme Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Ala-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Arg-Pro-7-amido-4-methylcoumarin + H2O high activity Hordeum vulgare Arg-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Asn-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Asn-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Asp-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Asp-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 beta-casomorphin + H2O cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP Hordeum vulgare fragments
-
 ?
3.4.14.5 des-Pro2-bradykinin + H2O cleavage sites: RP-/-GFSPFR Hordeum vulgare fragments
-
 ?
3.4.14.5 Gln-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Gln-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Glu-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Glu-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 His-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare His-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Ile-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Ile-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare L-Thr-L-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Leu-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Leu-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Lys-Ala-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Lys-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Lys-Pro-7-amido-4-methylcoumarin + H2O best substrate Hordeum vulgare Lys-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Met-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Met-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 additional information substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin Hordeum vulgare ?
-
 ?
3.4.14.5 Phe-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Phe-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Pro-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Pro-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Ser-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Ser-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 substance P + H2O cleavage sites: RP-/-KP-/-QQFFGLM Hordeum vulgare substance P peptide fragments
-
 ?
3.4.14.5 Tyr-Pro-7-amido-4-methylcoumarin + H2O
-
 Hordeum vulgare Tyr-Pro + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 monomer 1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 dipeptidyl peptidase IV
-
 Hordeum vulgare
3.4.14.5 DPPIV
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 25
-
 assay at Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.5 1.8
-
 Phe-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 4.6
-
 Asp-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 10
-
 Glu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 10
-
 Gln-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 22
-
 Leu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 23
-
 Ala-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 35
-
 Asn-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 41
-
 His-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 41
-
 Pro-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 58
-
 Ser-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 60
-
 Gly-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 67
-
 Ile-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 71
-
 Arg-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 90
-
 Lys-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 92
-
 Lys-Ala-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 99
-
 Tyr-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 102
-
 Met-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
3.4.14.5 117
-
 L-Thr-L-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.2
-
-
 Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5.5 9
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.14.5 Hordeum vulgare isoelectric focusing
-
 3.6