BRENDA - Enzyme Database

Purification and characterization of barley dipeptidyl peptidase IV

Davy, A.; Thomsen, K.K.; Juliano, M.A.; Alves, L.C.; Svendsen, I.; Simpson, D.J.; Plant Physiol. 122, 425-432 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.5
4-(2-aminoethyl)benzenesulfonyl fluoride
i.e. AEBSF
Hordeum vulgare
3.4.14.5
Diprotin A
specific inhibition
Hordeum vulgare
3.4.14.5
Diprotin B
specific inhibition
Hordeum vulgare
3.4.14.5
additional information
no inhibition by EDTA, E-64, pepstatin A or leupeptin
Hordeum vulgare
3.4.14.5
PMSF
-
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.5
0.000071
-
Lys-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00009
-
Arg-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00034
-
Ala-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00065
-
Phe-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.0007
-
Ile-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00073
-
Tyr-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00093
-
L-Thr-L-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00103
-
His-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00109
-
Leu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.0011
-
Pro-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00126
-
Ser-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00138
-
Gln-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00217
-
Met-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00298
-
Glu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00324
-
Asn-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00374
-
Lys-Ala-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00688
-
Gly-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00888
-
Asp-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.5
85000
-
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE
Hordeum vulgare
3.4.14.5
105000
-
gel filtration
Hordeum vulgare
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.14.5
Hordeum vulgare
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.4.14.5
glycoprotein
the deglycosylated enzyme is still active
Hordeum vulgare
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.14.5
from green barley malt, more than 43000fold
Hordeum vulgare
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.4.14.5
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline
serine protease
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.5
seed
malt from green barley
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.14.5
additional information
-
purified enzyme
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.14.5
Ala-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ala-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Arg-Pro-7-amido-4-methylcoumarin + H2O
high activity
653506
Hordeum vulgare
Arg-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Asn-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Asn-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Asp-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Asp-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
beta-casomorphin + H2O
cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
des-Pro2-bradykinin + H2O
cleavage sites: RP-/-GFSPFR
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
Gln-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Gln-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Glu-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Glu-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Gly-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
His-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
His-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Ile-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ile-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
L-Thr-L-Pro + 7-amino-4-methylcoumarin
-
-
-
?
3.4.14.5
Leu-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Leu-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Lys-Ala-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Lys-Ala + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Lys-Pro-7-amido-4-methylcoumarin + H2O
best substrate
653506
Hordeum vulgare
Lys-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Met-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Met-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
additional information
substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin
653506
Hordeum vulgare
?
-
653506
Hordeum vulgare
?
3.4.14.5
Phe-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Phe-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Pro-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Pro-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Ser-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ser-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
substance P + H2O
cleavage sites: RP-/-KP-/-QQFFGLM
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
Tyr-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Tyr-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.14.5
monomer
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE
Hordeum vulgare
Synonyms
EC Number
Synonyms
Commentary
Organism
3.4.14.5
dipeptidyl peptidase IV
-
Hordeum vulgare
3.4.14.5
DPPIV
-
Hordeum vulgare
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.14.5
25
-
assay at
Hordeum vulgare
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.5
1.8
-
Phe-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
4.6
-
Asp-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
10
-
Glu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
10
-
Gln-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
22
-
Leu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
23
-
Ala-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
35
-
Asn-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
41
-
His-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
41
-
Pro-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
58
-
Ser-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
60
-
Gly-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
67
-
Ile-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
71
-
Arg-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
90
-
Lys-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
92
-
Lys-Ala-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
99
-
Tyr-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
102
-
Met-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
117
-
L-Thr-L-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.5
7.2
-
-
Hordeum vulgare
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.14.5
5.5
9
-
Hordeum vulgare
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.14.5
Hordeum vulgare
isoelectric focusing
-
3.6
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.5
4-(2-aminoethyl)benzenesulfonyl fluoride
i.e. AEBSF
Hordeum vulgare
3.4.14.5
Diprotin A
specific inhibition
Hordeum vulgare
3.4.14.5
Diprotin B
specific inhibition
Hordeum vulgare
3.4.14.5
additional information
no inhibition by EDTA, E-64, pepstatin A or leupeptin
Hordeum vulgare
3.4.14.5
PMSF
-
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.5
0.000071
-
Lys-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00009
-
Arg-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00034
-
Ala-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00065
-
Phe-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.0007
-
Ile-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00073
-
Tyr-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00093
-
L-Thr-L-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00103
-
His-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00109
-
Leu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.0011
-
Pro-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00126
-
Ser-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00138
-
Gln-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00217
-
Met-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00298
-
Glu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00324
-
Asn-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00374
-
Lys-Ala-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00688
-
Gly-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
0.00888
-
Asp-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.5
85000
-
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE
Hordeum vulgare
3.4.14.5
105000
-
gel filtration
Hordeum vulgare
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.4.14.5
glycoprotein
the deglycosylated enzyme is still active
Hordeum vulgare
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.14.5
from green barley malt, more than 43000fold
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.5
seed
malt from green barley
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.14.5
additional information
-
purified enzyme
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.14.5
Ala-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ala-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Arg-Pro-7-amido-4-methylcoumarin + H2O
high activity
653506
Hordeum vulgare
Arg-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Asn-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Asn-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Asp-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Asp-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
beta-casomorphin + H2O
cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
des-Pro2-bradykinin + H2O
cleavage sites: RP-/-GFSPFR
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
Gln-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Gln-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Glu-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Glu-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Gly-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
His-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
His-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Ile-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ile-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
L-Thr-L-Pro + 7-amino-4-methylcoumarin
-
-
-
?
3.4.14.5
Leu-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Leu-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Lys-Ala-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Lys-Ala + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Lys-Pro-7-amido-4-methylcoumarin + H2O
best substrate
653506
Hordeum vulgare
Lys-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Met-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Met-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
additional information
substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin
653506
Hordeum vulgare
?
-
653506
Hordeum vulgare
?
3.4.14.5
Phe-Pro-7-amido-4-methylcoumarin + H2O
low activity
653506
Hordeum vulgare
Phe-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Pro-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Pro-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
Ser-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Ser-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
3.4.14.5
substance P + H2O
cleavage sites: RP-/-KP-/-QQFFGLM
653506
Hordeum vulgare
fragments
-
653506
Hordeum vulgare
?
3.4.14.5
Tyr-Pro-7-amido-4-methylcoumarin + H2O
-
653506
Hordeum vulgare
Tyr-Pro + 7-amino-4-methylcoumarin
-
653506
Hordeum vulgare
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.14.5
monomer
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.14.5
25
-
assay at
Hordeum vulgare
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.5
1.8
-
Phe-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
4.6
-
Asp-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
10
-
Glu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
10
-
Gln-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
22
-
Leu-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
23
-
Ala-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
35
-
Asn-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
41
-
His-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
41
-
Pro-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
58
-
Ser-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
60
-
Gly-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
67
-
Ile-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
71
-
Arg-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
90
-
Lys-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
92
-
Lys-Ala-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
99
-
Tyr-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
102
-
Met-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
3.4.14.5
117
-
L-Thr-L-Pro-7-amido-4-methylcoumarin
pH 7.2, 25°C
Hordeum vulgare
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.5
7.2
-
-
Hordeum vulgare
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.14.5
5.5
9
-
Hordeum vulgare
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.14.5
Hordeum vulgare
isoelectric focusing
-
3.6