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Literature summary extracted from

  • Zhu, Y.L.; Pilon-Smits, E.A.H.; Jouanin, L.; Terry, N.
    Overexpression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance (1999), Plant Physiol., 119, 73-79.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.2.3 biotechnology overexpression of the enzyme in transgenic plants offers a promising strategy for the production of plants with superior heavy-metal phytoremediation capacity Brassica juncea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 overexpression in Brassica juncea plants via infection with Agrobacterium tumefaciens, under control of the 35S cauliflower mosaic virus promotor Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.3 additional information construction of transgenic plants by overexpression of the enzyme in the cytosol of Brassica juncea, i.e. Indian mustard, transgenic plants show 3fold increased ability in the shoot compared to the wild-type to accumulate and tolerate heavy metals, e.g. cadmium, for use in detoxification Escherichia coli
6.3.2.3 additional information construction of transgenic plants by overexpression of the Escherichia coli enzyme, encoded by gene gshII, in the cytosol of transgenic plants, the plants show 3fold increased ability in the shoot compared to the wild-type to accumulate and tolerate heavy metals, e.g. cadmium Brassica juncea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.3 Cd2+ transgenic plants overexpressing the enzyme from Escherichia coli show enhanced tolerance against cadmium, 3fold in the shoot Brassica juncea
6.3.2.3 additional information transgenic plants overexpressing the enzyme from Escherichia coli show enhanced salt tolerance Brassica juncea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.3 38000
-
x * 38000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Escherichia coli
-
ADP + phosphate + glutathione
-
ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Brassica juncea
-
ADP + phosphate + glutathione
-
ir

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Brassica juncea
-
indian mustard
-
6.3.2.3 Escherichia coli
-
gene gshII
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.3 root
-
Brassica juncea
-
6.3.2.3 seed
-
Brassica juncea
-
6.3.2.3 shoot
-
Brassica juncea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
Escherichia coli ADP + phosphate + glutathione
-
ir
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
Brassica juncea ADP + phosphate + glutathione
-
ir

Subunits

EC Number Subunits Comment Organism
6.3.2.3 ? x * 38000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.2.3 Glutathione synthetase
-
Escherichia coli
6.3.2.3 Glutathione synthetase
-
Brassica juncea
6.3.2.3 GS
-
Escherichia coli
6.3.2.3 GS
-
Brassica juncea

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.3 ATP
-
Escherichia coli
6.3.2.3 ATP
-
Brassica juncea