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Literature summary extracted from

  • Allina, S.M.; Pri-Hadash, A.; Theilmann, D.A.; Ellis, B.E.; Douglas, C.J.
    4-Coumarate:coenzyme A ligase in hybrid poplar. Properties of native enzymes, cDNA cloning, and analysis of recombinant enzymes (1998), Plant Physiol., 116, 743-754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Sf9 cells using the baculovirus system Populus trichocarpa x Populus deltoides

General Stability

EC Number General Stability Organism
6.2.1.12 purified enzyme is very labile, no stabilizer found Populus trichocarpa x Populus deltoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.08
-
 4-coumarate room temperature Populus trichocarpa x Populus deltoides
6.2.1.12 0.102
-
 ferulate room temperature Populus trichocarpa x Populus deltoides
6.2.1.12 1.048
-
 cinnamate room temperature Populus trichocarpa x Populus deltoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 60000
-
 SDS-PAGE Populus trichocarpa x Populus deltoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Populus trichocarpa x Populus deltoides plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Populus trichocarpa x Populus deltoides
-
 hybid poplar, clone H1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 native and recombinant enzymes Populus trichocarpa x Populus deltoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 0.013
-
 purified leaf enzyme, room temperature Populus trichocarpa x Populus deltoides
6.2.1.12 0.13
-
 purified cell culture enzyme, room temperature Populus trichocarpa x Populus deltoides
6.2.1.12 0.33
-
 purified xylem enzyme, room temperature Populus trichocarpa x Populus deltoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Populus trichocarpa x Populus deltoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Populus trichocarpa x Populus deltoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Populus trichocarpa x Populus deltoides AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA roughly 30% of activity Populus trichocarpa x Populus deltoides AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA roughly 60% of activity Populus trichocarpa x Populus deltoides AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information inactive against sinapic acid Populus trichocarpa x Populus deltoides ?
-
 ?