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Literature summary extracted from

  • Andriotis, V.M.; Ross, J.D.
    Isolation and characterisation of phytase from dormant Corylus avellana seeds (2003), Phytochemistry, 64, 689-699.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 CuSO4 2.5 mM, 70% inhibition Corylus avellana
3.1.3.8 F- 1 mM, 49% inhibition. 2.5 mM, 61% inhibition Corylus avellana
3.1.3.8 Fe3+ 2.5 mM, 68% inhibition Corylus avellana
3.1.3.8 MgCl2 2.5 mM, 20% inhibition Corylus avellana
3.1.3.8 MnCl2 2.5 mM, 17% inhibition Corylus avellana
3.1.3.8 Na2MoO4 2.5 mM, 87% inhibition Corylus avellana
3.1.3.8 Na3VO4 0.1 m, 79% inhibition. 2.5 mM, 90% inhibition Corylus avellana
3.1.3.8 ZnSO4 2.5 mM, 59% inhibition Corylus avellana
3.1.3.26 CuSO4 2.5 mM, 70% inhibition Corylus avellana
3.1.3.26 F- 1 mM, 49% inhibition. 2.5 mM, 61% inhibition Corylus avellana
3.1.3.26 Fe3+ 2.5 mM, 68% inhibition Corylus avellana
3.1.3.26 MgCl2 2.5 mM, 20% inhibition Corylus avellana
3.1.3.26 MnCl2 2.5 mM, 17% inhibition Corylus avellana
3.1.3.26 Na2MoO4 2.5 mM, 87% inhibition Corylus avellana
3.1.3.26 Na3VO4 0.1 m, 79% inhibition. 2.5 mM, 90% inhibition Corylus avellana
3.1.3.26 ZnSO4 2.5 mM, 59% inhibition Corylus avellana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.16
-
 myo-inositol hexakisphosphate pH 5.0 Corylus avellana
3.1.3.8 0.55
-
 5'-ATP
-
 Corylus avellana
3.1.3.8 1.1
-
 D-fructose 1,6-diphosphate
-
 Corylus avellana
3.1.3.8 1.2
-
 D-ribose 5-phosphate
-
 Corylus avellana
3.1.3.8 1.6
-
 1-naphthyl phosphate
-
 Corylus avellana
3.1.3.8 2.2
-
 4-nitrophenyl phosphate pH 5.6 Corylus avellana
3.1.3.8 2.3
-
 D-glucose 6-phosphate
-
 Corylus avellana
3.1.3.8 2.4
-
 phosphate
-
 Corylus avellana
3.1.3.26 0.16
-
 myo-inositol hexakisphosphate pH 5.0 Corylus avellana
3.1.3.26 0.55
-
 5'-ATP
-
 Corylus avellana
3.1.3.26 1.1
-
 D-fructose 1,6-diphosphate
-
 Corylus avellana
3.1.3.26 1.2
-
 D-ribose 5-phosphate
-
 Corylus avellana
3.1.3.26 1.6
-
 1-naphthyl phosphate
-
 Corylus avellana
3.1.3.26 2.2
-
 4-nitrophenyl phosphate pH 5.6 Corylus avellana
3.1.3.26 2.3
-
 D-glucose 6-phosphate
-
 Corylus avellana
3.1.3.26 2.4
-
 phosphate
-
 Corylus avellana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 71000
-
 1 * 71000, SDS-PAGE Corylus avellana
3.1.3.8 72000
-
 gel filtration Corylus avellana
3.1.3.26 71000
-
 1 * 71000, SDS-PAGE Corylus avellana
3.1.3.26 72000
-
 gel filtration Corylus avellana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Corylus avellana
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Corylus avellana
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein
-
 Corylus avellana
3.1.3.26 glycoprotein
-
 Corylus avellana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Corylus avellana
3.1.3.26
-
 Corylus avellana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 cotyledon of dormant seed Corylus avellana
-
3.1.3.8 seed dormant Corylus avellana
-
3.1.3.26 cotyledon of dormant seed Corylus avellana
-
3.1.3.26 seed dormant Corylus avellana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 6.56
-
-
 Corylus avellana
3.1.3.26 6.56
-
-
 Corylus avellana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O 130% of the activity with myo-inositol hexakisphosphate Corylus avellana 1-naphthol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O 101% of the activity with myo-inositol hexakisphosphate Corylus avellana 4-nitrophenol + phosphate
-
 ?
3.1.3.8 5'-ATP + H2O 250% of the activity with myo-inositol hexakisphosphate Corylus avellana ?
-
 ?
3.1.3.8 D-fructose 1,6-diphosphate + H2O 146% of the activity with myo-inositol hexakisphosphate Corylus avellana ?
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O 78.5% of the activity with myo-inositol hexakisphosphate Corylus avellana D-glucose + phosphate
-
 ?
3.1.3.8 D-ribose 5-phosphate + H2O 50% of the activity with myo-inositol hexakisphosphate Corylus avellana D-ribose + phosphate
-
 ?
3.1.3.8 diphosphate + H2O 438.5% of the activity with myo-inositol hexakisphosphate Corylus avellana 2 phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O step-wise hydrolysis of myo-inositol hexakisphosphate Corylus avellana ? + phosphate
-
 ?
3.1.3.26 1-naphthyl phosphate + H2O 130% of the activity with myo-inositol hexakisphosphate Corylus avellana 1-naphthol + phosphate
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O 101% of the activity with myo-inositol hexakisphosphate Corylus avellana 4-nitrophenol + phosphate
-
 ?
3.1.3.26 5'-ATP + H2O 250% of the activity with myo-inositol hexakisphosphate Corylus avellana ?
-
 ?
3.1.3.26 D-fructose 1,6-diphosphate + H2O 146% of the activity with myo-inositol hexakisphosphate Corylus avellana ?
-
 ?
3.1.3.26 D-glucose 6-phosphate + H2O 78.5% of the activity with myo-inositol hexakisphosphate Corylus avellana D-glucose + phosphate
-
 ?
3.1.3.26 D-ribose 5-phosphate + H2O 50% of the activity with myo-inositol hexakisphosphate Corylus avellana D-ribose + phosphate
-
 ?
3.1.3.26 diphosphate + H2O 438.5% of the activity with myo-inositol hexakisphosphate Corylus avellana 2 phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O step-wise hydrolysis of myo-inositol hexakisphosphate Corylus avellana ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 71000, SDS-PAGE Corylus avellana
3.1.3.26 monomer 1 * 71000, SDS-PAGE Corylus avellana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Corylus avellana
3.1.3.26 5
-
-
 Corylus avellana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3.5 6.5 50% of maximal activity at pH 3.5 and pH 6.5 Corylus avellana
3.1.3.26 3.5 6.5 50% of maximal activity at pH 3.5 and pH 6.5 Corylus avellana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.8 0.205
-
 F-
-
 Corylus avellana
3.1.3.26 0.205
-
 F-
-
 Corylus avellana